Linked Life Data

17644524

Source:http://linkedlifedata.com/resource/pubmed/id/17644524

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
38
pubmed:dateCreated
2007-9-14
pubmed:abstractText
The par2 locus of Escherichia coli plasmid pB171 encodes two trans-acting proteins, ParA and ParB, and two cis-acting sites, parC1 and parC2, to which ParB binds cooperatively. ParA is related to MinD and oscillates in helical structures and thereby positions ParB/parC-carrying plasmids regularly over the nucleoid. ParB ribbon-helix-helix dimers bind cooperatively to direct repeats in parC1 and parC2. Using four different assays we obtain solid evidence that ParB can pair parC1- and parC2-encoding DNA fragments in vitro. Convincingly, electron microscopy revealed that ParB mediates binary pairing of parC fragments. In addition to binary complexes, ParB mediated the formation of higher order complexes consisting of several DNA fragments joined by ParB at centromere site parC. N-terminal truncated versions of ParB still possessing specific DNA binding activity were incompetent in pairing, hence identifying the N terminus of ParB as a requirement for ParB-mediated centromere pairing. These observations suggest that centromere pairing is an important intermediate step in plasmid partitioning mediated by the common type I loci.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
21
pubmed:volume
282
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
28216-25
pubmed:meshHeading
pubmed-meshheading:17644524-Amino Acid Motifs, pubmed-meshheading:17644524-Amino Acid Sequence, pubmed-meshheading:17644524-Base Sequence, pubmed-meshheading:17644524-Centromere, pubmed-meshheading:17644524-DNA, pubmed-meshheading:17644524-Dimerization, pubmed-meshheading:17644524-Dose-Response Relationship, Drug, pubmed-meshheading:17644524-Electrons, pubmed-meshheading:17644524-Endodeoxyribonucleases, pubmed-meshheading:17644524-Escherichia coli, pubmed-meshheading:17644524-Escherichia coli Proteins, pubmed-meshheading:17644524-Exodeoxyribonucleases, pubmed-meshheading:17644524-Molecular Sequence Data, pubmed-meshheading:17644524-Oscillometry, pubmed-meshheading:17644524-Plasmids, pubmed-meshheading:17644524-Protein Structure, Tertiary, pubmed-meshheading:17644524-Spectrometry, Fluorescence
pubmed:year
2007
pubmed:articleTitle
Centromere pairing by a plasmid-encoded type I ParB protein.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't