Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2008-3-11
pubmed:abstractText
A vaccine formula comprised of five recombinant human intra-acrosomal sperm proteins was inoculated into female monkeys to test whether specific antibodies to each component immunogen could be elicited in sera and whether antibodies elicited by the vaccine affected in vitro fertilization. Acrosomal proteins, ESP, SLLP-1, SAMP 32, SP-10 and SAMP 14, were expressed with his-tags, purified by nickel affinity chromatography and adsorbed to aluminum hydroxide. Five female cynomolgus monkeys were inoculated intramuscularly three times at monthly intervals. All five monkeys developed both IgG and IgA serum responses to each recombinant immunogen on Western blots. Each serum stained the acrosome of human sperm and bound to the cognate native protein on Western blots of human sperm extracts. By ELISA, all monkeys developed IgG to each immunogen, with the highest average absorbance values to ESP, SAMP 32 and SP-10, followed by lower values for SLLP-1 and SAMP 14. IgA was also generated to each component immunogen with the highest average absorbance values to SLLP-1 and SP-10. For antigens that induced an IgA response, the duration of the IgA response was longer than the IgG response to the same antigens. This study supports the concept that a multivalent contraceptive vaccine may be administered to female primates evoking both peripheral (IgG) and mucosal (IgA) responses to each component immunogen following an intramuscular route of inoculation with a mild adjuvant, aluminum hydroxide, approved for human use.
pubmed:grant
http://linkedlifedata.com/resource/pubmed/grant/D43 TW000654-05, http://linkedlifedata.com/resource/pubmed/grant/D43 TW000654-06, http://linkedlifedata.com/resource/pubmed/grant/D43 TW000654-07, http://linkedlifedata.com/resource/pubmed/grant/D43 TW000654-08, http://linkedlifedata.com/resource/pubmed/grant/D43 TW000654-09, http://linkedlifedata.com/resource/pubmed/grant/D43 TW000654-10, http://linkedlifedata.com/resource/pubmed/grant/D43 TW000654-10S1, http://linkedlifedata.com/resource/pubmed/grant/D43 TW000654-11, http://linkedlifedata.com/resource/pubmed/grant/D43 TW000654-12, http://linkedlifedata.com/resource/pubmed/grant/D43 TW000654-13, http://linkedlifedata.com/resource/pubmed/grant/P51 RR000166-38S10110, http://linkedlifedata.com/resource/pubmed/grant/P51 RR000166-400110, http://linkedlifedata.com/resource/pubmed/grant/U54 HD029099-060003, http://linkedlifedata.com/resource/pubmed/grant/U54 HD029099-070003, http://linkedlifedata.com/resource/pubmed/grant/U54 HD029099-08, http://linkedlifedata.com/resource/pubmed/grant/U54 HD029099-080003, http://linkedlifedata.com/resource/pubmed/grant/U54 HD029099-09, http://linkedlifedata.com/resource/pubmed/grant/U54 HD029099-090003, http://linkedlifedata.com/resource/pubmed/grant/U54 HD029099-10, http://linkedlifedata.com/resource/pubmed/grant/U54 HD029099-100003, http://linkedlifedata.com/resource/pubmed/grant/U54 HD029099-10S1, http://linkedlifedata.com/resource/pubmed/grant/U54 HD029099-10S10003, http://linkedlifedata.com/resource/pubmed/grant/U54 HD029099-10S2, http://linkedlifedata.com/resource/pubmed/grant/U54 HD029099-10S20003, http://linkedlifedata.com/resource/pubmed/grant/U54 HD029099-10S3, http://linkedlifedata.com/resource/pubmed/grant/U54 HD29099
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-10321806, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-10545912, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-11870081, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-12606493, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-12773409, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-12788941, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-15759005, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-1591355, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-1868140, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-2182290, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-2310816, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-2337631, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-2453766, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-581665, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-6767627, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-7202745, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-7536050, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-7625671, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-7888499, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-7926798, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-8027282, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-8447157, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-8573340, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-8779443, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-8879510, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-8882296, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-8962640, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-9359711, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-9364680, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-9369161, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-9421797, http://linkedlifedata.com/resource/pubmed/commentcorrection/17643494-9554262
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/ACRV1 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin A, http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G, http://linkedlifedata.com/resource/pubmed/chemical/Isoantigens, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins, http://linkedlifedata.com/resource/pubmed/chemical/SPACA1 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/SPACA3 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/SPACA4 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Seminal Plasma Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Contraceptive
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0165-0378
pubmed:author
pubmed:issnType
Print
pubmed:volume
77
More...