Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1992-2-18
pubmed:abstractText
Monoclonal antibodies, each recognizing interleukin 2 receptor (IL-2R) alpha, or beta, were used to see the regulatory mechanisms of the expressions on leukemic cells from a patient with T4 chronic lymphocytic leukemia (T4-CLL). Cells from this patient expressed only IL-2R beta, and the expression was enhanced by medium cultivation. IL-1 enhanced the expression of not only IL-2R beta but also IL-2R alpha on the cells. Binding studies using 125I-IL-2 showed the presence of an intermediate receptor (734 sites/cell, Kd = 1.2 nM) and a few high-affinity receptor (172 sites/cell, Kd = 132 pM) on cells cultured with IL-1. IL-2 and IL-1 synergistically promoted the proliferation of the cells, suggesting that the induced IL-2R was functional. In addition, anti-IL-1 antibodies inhibited IL-2R beta expression by cultured cells, suggesting that it was dependent on IL-1 produced by the leukemic cells. These findings suggested that IL-1 might enhance the expression of IL-2R beta in a subset of human T cells, and implied a role of IL-1 in the proliferation of the leukemic T cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0897-7194
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
289-95
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:1764265-Aged, pubmed-meshheading:1764265-Aged, 80 and over, pubmed-meshheading:1764265-Antibodies, Monoclonal, pubmed-meshheading:1764265-Binding Sites, pubmed-meshheading:1764265-Blotting, Northern, pubmed-meshheading:1764265-Cell Division, pubmed-meshheading:1764265-Cell Membrane, pubmed-meshheading:1764265-Cytokines, pubmed-meshheading:1764265-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:1764265-Female, pubmed-meshheading:1764265-Flow Cytometry, pubmed-meshheading:1764265-Humans, pubmed-meshheading:1764265-Interleukin-1, pubmed-meshheading:1764265-Interleukin-2, pubmed-meshheading:1764265-Leukemia, Prolymphocytic, T-Cell, pubmed-meshheading:1764265-Phytohemagglutinins, pubmed-meshheading:1764265-Receptors, Interleukin-2, pubmed-meshheading:1764265-Tumor Cells, Cultured
pubmed:year
1991
pubmed:articleTitle
Regulatory mechanisms for the expression of a functional beta chain of interleukin 2 receptor on T4 chronic lymphocytic leukemia cells.
pubmed:affiliation
Second Department of Internal Medicine, Kumamoto University Medical School, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't