Source:http://linkedlifedata.com/resource/pubmed/id/17641286
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2007-7-20
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| pubmed:databankReference | |
| pubmed:abstractText |
Two prolactin receptors (PRLRs) encoded by two different genes were identified in the fugu and zebrafish genomes but not in the genomes of other vertebrates. Subsequently, two cDNA sequences corresponding to two PRLRs were identified in black seabream and Nile tilapia. Phylogenetic analysis of PRLR sequences in various vertebrates indicated that the coexistence of two PRLRs in a single species is a unique phenomenon in teleosts. Both PRLRs in teleosts (the classical one named as PRLR1, the newly identified one as PRLR2) resemble the long-form mammalian PRLRs. However, despite their overall structural similarities, the two PRLR subtypes in fish share very low amino acid similarities (about 30%), mainly due to differences in the intracellular domain. In particular, the Box 2 region and some intracellular tyrosine residues are missing in PRLR2. Tissue distribution study by real-time PCR in black seabream (sb) revealed that both receptors (sbPRLR1 and sbPRLR2) are widely expressed in different tissues. In gill, the expression level of sbPRLR2 is much higher than that of sbPRLR1. In the intestine, the expression of sbPRLR1 is higher than that of sbPRLR2. The expression levels of both receptors are relatively low in most other tissues, with sbPRLR1 generally higher than sbPRLR2. The sbPRLR1 and sbPRLR2 were functionally expressed in cultured human embryonic kidney 293 cells. Both receptors can activate the beta-casein and c-fos promoters; however, only sbPRLR1 but not sbPRLR2 can activate the Spi promoter upon receptor stimulation in a ligand-specific manner. These results indicate that both receptors share some common functions but are distinctly different from each other in mobilizing post-receptor events. When challenged with different steroid hormones, the two PRLRs exhibited very different gene expression patterns in the seabream kidney. The sbPRLR1 expression was up-regulated by estradiol and cortisol, whereas testosterone had no significant effect. For sbPRLR2, its expression was down-regulated by estradiol and testosterone, while cortisol exerted no significant effect. The 5'-flanking regions of the sbPRLR1 and sbPRLR2 genes were cloned and the promoter activities were studied in transfected GAKS cells in the absence or presence of different steroid hormones. The results of the promoter studies were in general agreement with the in vivo hormonal regulation of gene expression results. The sbPRLR1 gene promoter activity was activated by estradiol and cortisol, but not by testosterone. In contrast, the sbPRLR2 gene promoter activity was inhibited by estradiol, cortisol, and testosterone.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Estradiol,
http://linkedlifedata.com/resource/pubmed/chemical/Hormones,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrocortisone,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Prolactin,
http://linkedlifedata.com/resource/pubmed/chemical/Testosterone
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| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
0022-0795
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
194
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
373-92
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:17641286-5' Flanking Region,
pubmed-meshheading:17641286-Amino Acid Sequence,
pubmed-meshheading:17641286-Animals,
pubmed-meshheading:17641286-Base Sequence,
pubmed-meshheading:17641286-Binding Sites,
pubmed-meshheading:17641286-Estradiol,
pubmed-meshheading:17641286-Female,
pubmed-meshheading:17641286-Gene Expression,
pubmed-meshheading:17641286-Hormones,
pubmed-meshheading:17641286-Humans,
pubmed-meshheading:17641286-Hydrocortisone,
pubmed-meshheading:17641286-Kidney,
pubmed-meshheading:17641286-Male,
pubmed-meshheading:17641286-Molecular Sequence Data,
pubmed-meshheading:17641286-Promoter Regions, Genetic,
pubmed-meshheading:17641286-RNA, Messenger,
pubmed-meshheading:17641286-Receptors, Prolactin,
pubmed-meshheading:17641286-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:17641286-Sea Bream,
pubmed-meshheading:17641286-Sequence Analysis, DNA,
pubmed-meshheading:17641286-Signal Transduction,
pubmed-meshheading:17641286-Takifugu,
pubmed-meshheading:17641286-Testosterone,
pubmed-meshheading:17641286-Tilapia,
pubmed-meshheading:17641286-Transfection,
pubmed-meshheading:17641286-Zebrafish
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| pubmed:year |
2007
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| pubmed:articleTitle |
The presence of two distinct prolactin receptors in seabream with different tissue distribution patterns, signal transduction pathways and regulation of gene expression by steroid hormones.
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| pubmed:affiliation |
Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing 400715, China.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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