Linked Life Data

17640404

Source:http://linkedlifedata.com/resource/pubmed/id/17640404

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2007-7-20
pubmed:abstractText
Previous studies of rod photoreceptors in vivo have employed a paired-flash electroretinographic (ERG) technique to determine rod response properties. To test whether absence versus presence of the ERG b-wave affects the photoreceptor response derived by the paired-flash method, we examined paired-flash-derived responses obtained from nob mice, a mutant strain with a defect in signal transduction between photoreceptors and ON bipolar cells that causes a lack of the b-wave. Normal littermates of the nob mice served as controls. The normalized amplitude-intensity relation of the derived response determined in nob mice at the near-peak time of 86 ms was similar to that determined for the controls. The full time course of the derived rod response was obtained for test flash strengths ranging from 0.11 to 17.38 scotopic cd s m(-2) (sc cd s m(-2)). Time-course data obtained from nob and control mice exhibited significant but generally modest differences. With saturating test flash strengths, half-recovery times for the derived response of nob versus control mice differed by approximately 60 ms or less about the combined (nob and control) average respective values. Time course data also were obtained before versus after intravitreal injection of L-2-amino-4-phosphonobutyrate (APB) (which blocks transmission from photoreceptors to depolarizing bipolar cells) and of cis 2,3-piperidine dicarboxylic acid (PDA) (which blocks transmission to OFF bipolar cells, and to horizontal, amacrine and ganglion cells). Neither APB nor PDA substantially affected derived responses obtained from nob or control mice. The results provide quantitative information on the effect of b-wave removal on the paired-flash-derived response in mouse. They argue against a substantial skewing effect of the b-wave on the paired-flash-derived response obtained in normal mice and are consistent with the notion that, to good approximation, this derived response represents the isolated flash response of the photoreceptors in both nob and normal mice.
pubmed:grant
http://linkedlifedata.com/resource/pubmed/grant/EY016094, http://linkedlifedata.com/resource/pubmed/grant/EY01792, http://linkedlifedata.com/resource/pubmed/grant/EY05494, http://linkedlifedata.com/resource/pubmed/grant/EY06671, http://linkedlifedata.com/resource/pubmed/grant/EY07551, http://linkedlifedata.com/resource/pubmed/grant/MH075791, http://linkedlifedata.com/resource/pubmed/grant/P30 EY001792-30, http://linkedlifedata.com/resource/pubmed/grant/P30 EY007551-19, http://linkedlifedata.com/resource/pubmed/grant/P30 EY007551-20, http://linkedlifedata.com/resource/pubmed/grant/P30 EY007551-21, http://linkedlifedata.com/resource/pubmed/grant/P30 EY007551-24, http://linkedlifedata.com/resource/pubmed/grant/R01 EY005494-23, http://linkedlifedata.com/resource/pubmed/grant/R01 EY006671-13, http://linkedlifedata.com/resource/pubmed/grant/R01 EY006671-14, http://linkedlifedata.com/resource/pubmed/grant/R01 EY006671-15, http://linkedlifedata.com/resource/pubmed/grant/R01 EY016094-01A1
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0952-5238
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
141-9
pubmed:dateRevised
2011-5-4
pubmed:meshHeading
pubmed:articleTitle
Test of the paired-flash electroretinographic method in mice lacking b-waves.
pubmed:affiliation
Lions of Illinois Eye Research Institute, Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, College of Medicine, Chicago, Illinois 60612, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural