Source:http://linkedlifedata.com/resource/pubmed/id/17639959
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2007-7-20
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| pubmed:abstractText |
A monocrotophos [dimethyl (E)-1-2-methylcarbamoylvinylphosphate or MCP] -degrading strain named as M-1 was isolated from sludge collected from the wastewater treatment pool of a pesticide factory and identified as Paracoccus sp. according to its morphology and biochemical properties and 16S rDNA sequence analysis. Using MCP as a sole carbon source, M-1 was able to degrade MCP(100 mg x L(-1)) by 92.47% in 24 h. The key enzyme(s) involved in the initial biodegradation of monocrotophos in M-1 was shown to be constitutively expressed cytosolic proteins and showed the greatest activity at pH 8.0 and 25 degrees C, with its Michaelis-Mentn's constant (K(m)) and maximum degradation rate (V(max)) of 0.29 micromol x mL(-1) and 682.12 micromol (min x mg)(-1) respectively. This degrading enzyme(s) was sensitive to high temperature, but kept high activity under alkaline conditions.
|
| pubmed:language |
chi
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
|
| pubmed:issn |
0250-3301
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
28
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
908-12
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| pubmed:meshHeading | |
| pubmed:year |
2007
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| pubmed:articleTitle |
[Isolation of a monocrotophos-degrading bacterial strain and characterization of enzymatic degradation].
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| pubmed:affiliation |
Key Laboratory of Agricultural Environment Microbiological Engineering, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China. jiakaizhi@yahoo.com.cn
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| pubmed:publicationType |
Journal Article,
English Abstract,
Research Support, Non-U.S. Gov't
|