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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
14
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pubmed:dateCreated |
2007-7-19
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pubmed:abstractText |
Toward the goal of developing an optical imaging contrast agent that will enable surgeons to intraoperatively distinguish cancer foci from adjacent normal tissue, we developed a chlorotoxin:Cy5.5 (CTX:Cy5.5) bioconjugate that emits near-IR fluorescent signal. The probe delineates malignant glioma, medulloblastoma, prostate cancer, intestinal cancer, and sarcoma from adjacent non-neoplastic tissue in mouse models. Metastatic cancer foci as small as a few hundred cells were detected in lymph channels. Specific binding to cancer cells is facilitated by matrix metalloproteinase-2 (MMP-2) as evidenced by reduction of CTX:Cy5.5 binding in vitro and in vivo by a pharmacologic blocker of MMP-2 and induction of CTX:Cy5.5 binding in MCF-7 cells following transfection with a plasmid encoding MMP-2. Mouse studies revealed that CTX:Cy5.5 has favorable biodistribution and toxicity profiles. These studies show that CTX:Cy5.5 has the potential to fundamentally improve intraoperative detection and resection of malignancies.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0008-5472
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pubmed:author |
pubmed-author:BahramiS-BahramSB,
pubmed-author:EllenbogenRichard GRG,
pubmed-author:GabikianPatrikP,
pubmed-author:GradyWilliam MWM,
pubmed-author:GreenbergNorman MNM,
pubmed-author:HackmanRobert CRC,
pubmed-author:HansenStacey JSJ,
pubmed-author:KusumaYumikoY,
pubmed-author:KwokDeborahD,
pubmed-author:MunozNina MNM,
pubmed-author:OlsonJames MJM,
pubmed-author:RavanpayAli CAC,
pubmed-author:StroudMark RMR,
pubmed-author:SzeRaymond WRW,
pubmed-author:VeisehMandanaM,
pubmed-author:VeisehOmidO,
pubmed-author:ZhangMiqinM
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pubmed:issnType |
Print
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pubmed:day |
15
|
|
pubmed:volume |
67
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6882-8
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pubmed:dateRevised |
2007-12-3
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pubmed:meshHeading |
pubmed-meshheading:17638899-Animals,
pubmed-meshheading:17638899-Brain Neoplasms,
pubmed-meshheading:17638899-Carbocyanines,
pubmed-meshheading:17638899-Fluorescent Dyes,
pubmed-meshheading:17638899-Glioma,
pubmed-meshheading:17638899-Humans,
pubmed-meshheading:17638899-Matrix Metalloproteinase 2,
pubmed-meshheading:17638899-Mice,
pubmed-meshheading:17638899-Microscopy, Fluorescence,
pubmed-meshheading:17638899-Neoplasms,
pubmed-meshheading:17638899-Neovascularization, Pathologic,
pubmed-meshheading:17638899-Photons,
pubmed-meshheading:17638899-Rats,
pubmed-meshheading:17638899-Scorpion Venoms
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pubmed:year |
2007
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pubmed:articleTitle |
Tumor paint: a chlorotoxin:Cy5.5 bioconjugate for intraoperative visualization of cancer foci.
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pubmed:affiliation |
Clinical Research Division, Fred Hutchinson Cancer Research Center, University of Washington, 1100 Fairview Avenue N., Seattle, WA 98109, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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