Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2007-7-19
pubmed:abstractText
Pro-protein convertases (PCs) are a family of serine proteases (furin, PC1/3, PC2, PACE4, PC4, PC5/6, PC7/8) responsible for post-translational processing and activation of inactive precursors of many regulatory proteins. Endometrial PC6 is critical for implantation in mice and for decidualization of human endometrial stromal cells (ESCs). This study investigated the endometrial expression of other PCs during the menstrual cycle and early pregnancy to elucidate potential redundancies. Furin, PC4, PACE4, and PC7 along with PC6 transcripts were detected in total endometrial RNA, whereas PC1 and PC2 transcription levels were negligible. Quantitative RT-PCR demonstrated highest levels of furin mRNA during menstruation and lowest levels during the proliferative phase. Furin protein was immunolocalized in endometrial luminal and glandular epithelia, stromal fibroblasts, endothelia, and leukocytes. PACE4 and PC7 proteins were also immunodetected in endometrial stroma and glands. Total furin, PC7, and PACE4 proteins were constitutive in both stromal and glandular compartments throughout the cycle and during first trimester pregnancy. Furthermore, Furin and PC7 transcription was unaltered during decidualization of ESCs in vitro in contrast to PC6 which is significantly up-regulated during decidualization. Thus, whereas PC6 is tightly regulated during endometrial preparation for implantation, furin, PACE4, and PC7 are constitutively expressed in human endometrium, but must be considered if PC6 is to be targeted for manipulation of fertility.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1470-1626
pubmed:author
pubmed:issnType
Print
pubmed:volume
133
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1189-97
pubmed:meshHeading
pubmed-meshheading:17636173-Blotting, Western, pubmed-meshheading:17636173-Cells, Cultured, pubmed-meshheading:17636173-Decidua, pubmed-meshheading:17636173-Embryo Implantation, pubmed-meshheading:17636173-Endometrium, pubmed-meshheading:17636173-Female, pubmed-meshheading:17636173-Furin, pubmed-meshheading:17636173-Gene Expression, pubmed-meshheading:17636173-Gene Expression Regulation, pubmed-meshheading:17636173-Humans, pubmed-meshheading:17636173-Immunohistochemistry, pubmed-meshheading:17636173-Menstrual Cycle, pubmed-meshheading:17636173-Pregnancy, pubmed-meshheading:17636173-Pregnancy Trimester, First, pubmed-meshheading:17636173-Proprotein Convertase 1, pubmed-meshheading:17636173-Proprotein Convertase 2, pubmed-meshheading:17636173-Proprotein Convertases, pubmed-meshheading:17636173-RNA, Messenger, pubmed-meshheading:17636173-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:17636173-Serine Endopeptidases, pubmed-meshheading:17636173-Stromal Cells, pubmed-meshheading:17636173-Subtilisins
pubmed:year
2007
pubmed:articleTitle
Pro-protein convertases (PCs) other than PC6 are not tightly regulated for implantation in the human endometrium.
pubmed:affiliation
Prince Henry's Institute of Medical Research, PO Box 5152, Clayton, Victoria 3168, Australia. claudia_freyer@hotmail.com
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't