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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2007-10-26
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pubmed:abstractText |
HIV-1 Nef is a multifunctional protein that exerts its activities through interactions with multiple cellular partners. Nef uses different domains and mechanisms to exert its functions including cell surface down-modulation of CD4 and MHC-I receptors and activation of the serine/threonine kinase PAK-2. We inserted tags at the C-terminus and proximal to the N-terminus of Nef and the effects on Nef's structure/function relationships were examined. We discovered significant defects in MHC-I down-modulation with the insertion of HA/FLAG tags at either region. We also found impaired PAK-2 activation with a C-terminal fusion with GFP. Interestingly, Nef-GFP and Nef-GH(7) induced MHC-I down-modulation, suggesting that the negative charge of the HA/FLAG tag could contribute to the observed defect. Together, these observations highlight elements of Nef's functional complexity and demonstrate previously unsuspected structural requirements for PAK-2 activation and MHC-1 down-modulation in Nef's flexible N- and C-terminal regions.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-10339411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-10375525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-10375528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-10477559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-10559302,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-10684310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-10707087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-10814565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-10892807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-11070003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-11160665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-11160719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-11264355,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-11264386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-11328823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-11406408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-11463741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-12032142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-12414957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-12526811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-13679604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-14737186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-15043211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-15542684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-15596859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-16237100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-16415008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-16760313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-16784224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-2014052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-2032289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-3118220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-7541845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-7982906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-8098729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-8108442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-8151761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-8612235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-8709288,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-8862424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-8955034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-9052838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-9351809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-9384576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-9450757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-9582271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-9620685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-9736718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-9811611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17632197-9971776
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0042-6822
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
368
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7-16
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pubmed:dateRevised |
2011-9-26
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pubmed:meshHeading |
pubmed-meshheading:17632197-Antigens, CD4,
pubmed-meshheading:17632197-Cell Line,
pubmed-meshheading:17632197-Down-Regulation,
pubmed-meshheading:17632197-HIV-1,
pubmed-meshheading:17632197-Histocompatibility Antigens Class I,
pubmed-meshheading:17632197-Humans,
pubmed-meshheading:17632197-Protein Kinase C,
pubmed-meshheading:17632197-Protein Structure, Secondary,
pubmed-meshheading:17632197-nef Gene Products, Human Immunodeficiency Virus
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pubmed:year |
2007
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pubmed:articleTitle |
Structural constraints on human immunodeficiency virus type 1 Nef function.
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pubmed:affiliation |
University of Texas Southwestern Medical Center at Dallas, TX 75390, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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