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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2007-10-9
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pubmed:abstractText |
As a critical step toward understanding the role of abnormal intracellular Ca(2+) release via the ryanodine receptor (RyR(2)) during the development of hypertension-induced cardiac hypertrophy and heart failure, this study examines two questions: 1) At what stage, if ever, in the development of hypertrophy and heart failure is RyR(2) hyperphosphorylated at Ser(2808)? 2) Does the spatial distribution of RyR(2) clusters change in failing hearts? Using a newly developed semiquantitative immunohistochemistry method and Western blotting, we measured phosphorylation of RyR(2) at Ser(2808) in the spontaneously hypertensive rat (SHR) at four distinct disease stages. A major finding is that hyperphosphorylation of RyR(2) at Ser(2808) occurred only at late-stage heart failure in SHR, but not in age-matched controls. Furthermore, the spacing between RyR(2) clusters was shortened in failing hearts, as predicted by quantitative model simulation to increase spontaneous Ca(2+) wave generation and arrhythmias.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0363-6135
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
293
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
H2409-17
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pubmed:dateRevised |
2011-9-22
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pubmed:meshHeading |
pubmed-meshheading:17630346-Animals,
pubmed-meshheading:17630346-Blotting, Western,
pubmed-meshheading:17630346-Calcium,
pubmed-meshheading:17630346-Cardiomegaly,
pubmed-meshheading:17630346-Computer Simulation,
pubmed-meshheading:17630346-Cyclic AMP-Dependent Protein Kinases,
pubmed-meshheading:17630346-Disease Models, Animal,
pubmed-meshheading:17630346-Disease Progression,
pubmed-meshheading:17630346-Heart Failure,
pubmed-meshheading:17630346-Hypertension,
pubmed-meshheading:17630346-Immunohistochemistry,
pubmed-meshheading:17630346-Male,
pubmed-meshheading:17630346-Models, Cardiovascular,
pubmed-meshheading:17630346-Myocardium,
pubmed-meshheading:17630346-Phosphorylation,
pubmed-meshheading:17630346-Rats,
pubmed-meshheading:17630346-Rats, Inbred SHR,
pubmed-meshheading:17630346-Rats, Inbred WKY,
pubmed-meshheading:17630346-Reproducibility of Results,
pubmed-meshheading:17630346-Ryanodine Receptor Calcium Release Channel,
pubmed-meshheading:17630346-Serine
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pubmed:year |
2007
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pubmed:articleTitle |
Phosphorylation of RyR2 and shortening of RyR2 cluster spacing in spontaneously hypertensive rat with heart failure.
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pubmed:affiliation |
Dept. of Internal Medicine, Univ. of Kentucky College of Medicine, 741 S. Limestone St., BBSRB, Rm. B255, Lexington, KY 40536-0509, USA. YeChen-Izu@uky.edu
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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