Source:http://linkedlifedata.com/resource/pubmed/id/17629738
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-3
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| pubmed:dateCreated |
2007-11-16
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| pubmed:abstractText |
Membrane channel connexin (Cx) forms gap junctions that are implicated in the homeostatic regulation of multicellular systems; thus, hematopoietic cells were assumed not to express Cxs. However, hematopoietic progenitors organize a multicellular system during the primitive stage; thus, the aim of the present study was to determine whether Cx32, a member of the Cx family, may function during the primitive steady-state hematopoiesis in the bone marrow (BM). First, the numbers of mononuclear cells in the peripheral blood and various hematopoietic progenitor compartments in the BM decreased in Cx32-knockout (KO) mice. Second, on the contrary, the number of primitive hematopoietic progenitor cells, specifically the Lin(-)/c-kit(+)/Scal(+ )fraction, the KSL progenitor cell compartment, also increased in Cx32-KO mice. Third, expression of Cx32 was detected in Lin(-)/c-kit(+) hematopoietic progenitor cells of wild-type mice (0.27% in the BM), whereas it was not detected in unfractionated wild-type BM cells. Furthermore, cell-cycle analysis of the fractionated KSL compartment from Cx32-KO BM showed a higher ratio in the G(2)/M fraction. Taken together, all these results imply that Cx32 is expressed solely in the primitive stem cell compartment, which maintains the stemness of the cells, i.e., being quiescent and noncycling; and once Cx32 is knocked out, these progenitor cells are expected to enter the cell cycle, followed by proliferation and differentiation for maintaining the number of peripheral blood cells.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Connexins,
http://linkedlifedata.com/resource/pubmed/chemical/Ion Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleotidyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-kit,
http://linkedlifedata.com/resource/pubmed/chemical/connexin 32,
http://linkedlifedata.com/resource/pubmed/chemical/lincosaminide...
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jun
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| pubmed:issn |
0022-2631
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
217
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
105-13
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| pubmed:dateRevised |
2009-11-19
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| pubmed:meshHeading |
pubmed-meshheading:17629738-Animals,
pubmed-meshheading:17629738-Blotting, Western,
pubmed-meshheading:17629738-Bone Marrow,
pubmed-meshheading:17629738-Cell Differentiation,
pubmed-meshheading:17629738-Cell Division,
pubmed-meshheading:17629738-Cell Proliferation,
pubmed-meshheading:17629738-Cells, Cultured,
pubmed-meshheading:17629738-Connexins,
pubmed-meshheading:17629738-Female,
pubmed-meshheading:17629738-Flow Cytometry,
pubmed-meshheading:17629738-G2 Phase,
pubmed-meshheading:17629738-Hematopoietic Stem Cells,
pubmed-meshheading:17629738-Ion Channels,
pubmed-meshheading:17629738-Male,
pubmed-meshheading:17629738-Mice,
pubmed-meshheading:17629738-Mice, Inbred C57BL,
pubmed-meshheading:17629738-Mice, Knockout,
pubmed-meshheading:17629738-Nucleotidyltransferases,
pubmed-meshheading:17629738-Proto-Oncogene Proteins c-kit
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| pubmed:year |
2007
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| pubmed:articleTitle |
Membrane channel connexin 32 maintains Lin(-)/c-kit(+) hematopoietic progenitor cell compartment: analysis of the cell cycle.
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| pubmed:affiliation |
Division of Cellular and Molecular Toxicology, Center for Biological Safety and Research, National Institute of Health Sciences, Tokyo, Japan. yokohira@nihs.go.jp
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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