Source:http://linkedlifedata.com/resource/pubmed/id/17628859
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
2007-8-1
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| pubmed:abstractText |
We developed IgH-transgenic rabbits carrying a productive VDJ-Cmu Tg and found the rabbits were B cell-deficient, with a 50-100% reduction in serum IgM and IgG levels. The bone marrow of newborn Tg rabbits contained severely reduced levels of preB cells and almost no B cells. The few preB cells present in the bone marrow were large, cycling cells that expressed the VDJ-Cmu Tg, indicating that the block in B cell development likely occurred at or before the transition from large (early) preB to small (late) preB cells. By immunoprecipitation, the Tg mu-chain paired with VpreB and lambda5, suggesting that the B cell deficiency is not due to an inability to form a preB cell receptor. Despite the block in B cell development, a few B cells, expressing predominantly endogenous mu-chains, began the second stage of development in GALT. B cells were localized in and beneath the follicle-associated epithelium of GALT prior to B cell follicle formation, suggesting to us that B cell follicle formation is initiated near the follicle-associated epithelium, possibly through contact with intestinal microbiota. These IgH-Tg rabbits should provide a useful model for studies of B cell development both in bone marrow and in GALT.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
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| pubmed:issn |
0014-2980
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
37
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2290-9
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| pubmed:dateRevised |
2007-12-3
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| pubmed:meshHeading |
pubmed-meshheading:17628859-Amino Acid Sequence,
pubmed-meshheading:17628859-Animals,
pubmed-meshheading:17628859-Animals, Genetically Modified,
pubmed-meshheading:17628859-B-Lymphocytes,
pubmed-meshheading:17628859-Cell Differentiation,
pubmed-meshheading:17628859-Flow Cytometry,
pubmed-meshheading:17628859-Fluorescent Antibody Technique,
pubmed-meshheading:17628859-Genes, Immunoglobulin Heavy Chain,
pubmed-meshheading:17628859-Immunity, Mucosal,
pubmed-meshheading:17628859-Immunoglobulin G,
pubmed-meshheading:17628859-Immunoglobulin M,
pubmed-meshheading:17628859-Immunoglobulin mu-Chains,
pubmed-meshheading:17628859-Immunohistochemistry,
pubmed-meshheading:17628859-Intestines,
pubmed-meshheading:17628859-Rabbits,
pubmed-meshheading:17628859-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:17628859-VDJ Exons
|
| pubmed:year |
2007
|
| pubmed:articleTitle |
B lymphocyte deficiency in IgH-transgenic rabbits.
|
| pubmed:affiliation |
Department of Microbiology and Immunology, Loyola University Chicago, Maywood, IL 60153, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
|