Linked Life Data

17627859

Source:http://linkedlifedata.com/resource/pubmed/id/17627859

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2007-8-6
pubmed:abstractText
A soluble protein complex P60 from the powdered nacre of Pinctada fucata was extracted and partially characterized. The biological activity of the P60 on pre-osteoblast cell line MC3T3-E1 and bone marrow stroma cells (MSCs) was investigated. The P60 protein from the decalcified powered nacre was solubilized with acetic acid and then purified by liquid chromatography. The P60 protein was a protein complex composed of several subunits with disulfide bridges. The known protein nacrein, and its two derivatives, N28 and N35, were included in the P60 protein complex. The most abundant amino acids in the P60 that account for 68.3% of the total residues are glycine (32.1%), aspartic acid (17.4%), alanine (13.6%), and glutamic acid (5.2%). The in vitro study of the crystallization showed that this protein complex could control the formation and size of calcium carbonate. The assay of biological activity of the P60 protein complex on the pre-osteoblast cell line MC3T3-E1 and MSCs suggested that the P60 could stimulate the formation of mineralized nodules.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1096-4959
pubmed:author
pubmed:issnType
Print
pubmed:volume
148
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
201-8
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Characterization and in vitro mineralization function of a soluble protein complex P60 from the nacre of Pinctada fucata.
pubmed:affiliation
School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't