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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-3
|
| pubmed:dateCreated |
1992-2-13
|
| pubmed:abstractText |
To estimate functional diffusion distances, the distribution of perfused capillaries was calculated in dog myocardium. A fluorescent dye was injected via a femoral vein in 6 anesthetized, open-chest dogs, and passed once through the coronary circulation (23 +/- 3 s). In 6 animals the dye circulated 4-20 min. In 6 animals the dye circulated for one pass following 2-3 min of asphyxia. The heart was then removed and frozen. Frozen sections from the left ventricule were cut, illuminated to excite the dye to fluoresce, and photographed. They were then stained by silver methenamine to mark all capillaries. The density of all capillaries was compared to that of capillaries containing fluorescent label. The distributions of capillaries were estimated by morphometry. In one pass of the normoxic coronary circulation, 66(+/- 4 SE)% of subepicardial and 60(+/- 4)% of subendocardial capillaries were detectably labeled. Their distribution approached a random pattern, and maximal distances to the nearest labeled capillary were lengthened by 50% compared to all capillaries. With multiple passes of the dye, or with asphyxia 76-79% of the capillaries were detectably labeled and their distribution approached the ordered pattern of the total capillary bed. We speculated that the unlabeled capillaries represented a spatially heterogeneous blood flow reserve.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0740-9451
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
7
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
77-108
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1762611-Animals,
pubmed-meshheading:1762611-Asphyxia,
pubmed-meshheading:1762611-Capillaries,
pubmed-meshheading:1762611-Coronary Circulation,
pubmed-meshheading:1762611-Coronary Vessels,
pubmed-meshheading:1762611-Dogs,
pubmed-meshheading:1762611-Hemodynamics,
pubmed-meshheading:1762611-Microcirculation,
pubmed-meshheading:1762611-Oxygen Consumption,
pubmed-meshheading:1762611-Perfusion,
pubmed-meshheading:1762611-Random Allocation,
pubmed-meshheading:1762611-Specimen Handling
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
Capillary recruitment and heterogeneity of perfused capillary distribution in dog myocardium.
|
| pubmed:affiliation |
Department of Physiology and Biophysics, UMDNJ Robert Wood Johnson Medical School, Piscataway 08854-5635.
|
| pubmed:publicationType |
Journal Article
|