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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 7
pubmed:dateCreated
2007-7-12
pubmed:databankReference
pubmed:abstractText
Two novel strains belonging to the phylum Bacteroidetes [formerly the Cytophaga-Flexibacter-Bacteroides (CFB) group], designated Gsoil 040(T) and Gsoil 052(T), were isolated from the soil of a ginseng field in Pocheon province, South Korea. A polyphasic approach was used to characterize the taxonomic position of the novel strains. Both strains were Gram-negative, aerobic, non-motile, non-spore-forming and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel isolates belong to the genus Chitinophaga but are clearly separated from the recognized species of this genus; gene sequence similarities between the novel isolates and type strains of recognized species ranged from 91.2 to 96.5 %. One exception was found; strain Gsoil 052(T) and the type strain of Chitinophaga filiformis had a gene sequence similarity of 99.6 % but had a DNA-DNA relatedness value of 38 %. Phenotypic and chemotaxonomic data (major menaquinone, MK-7; major fatty acids, iso-C(15 : 0) and C(16 : 1)omega5c; major hydroxy fatty acid, iso-C(17 : 0) 3-OH and major polyamine, homospermidine) supported the affiliation of both strains Gsoil 040(T) and Gsoil 052(T) to the genus Chitinophaga. The results of physiological and biochemical tests enabled the genotypic and phenotypic differentiation of the novel strains from the other recognized species of the genus Chitinophaga. Therefore, it is suggested that the new isolates represent two novel species, for which the names Chitinophaga ginsengisoli [corrected] sp. nov. [type strain Gsoil 040(T) (=KCTC 12654(T)=DSM 18108(T))] and Chitinophaga ginsengisoli sp. nov. [type strain Gsoil 052(T) (=KCTC 12592(T)=DSM 18017(T))] are proposed.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1466-5026
pubmed:author
pubmed:issnType
Print
pubmed:volume
57
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1396-401
pubmed:dateRevised
2009-3-3
pubmed:meshHeading
pubmed-meshheading:17625164-Aerobiosis, pubmed-meshheading:17625164-Bacteroidetes, pubmed-meshheading:17625164-DNA, Bacterial, pubmed-meshheading:17625164-DNA, Ribosomal, pubmed-meshheading:17625164-Fatty Acids, pubmed-meshheading:17625164-Genes, rRNA, pubmed-meshheading:17625164-Korea, pubmed-meshheading:17625164-Locomotion, pubmed-meshheading:17625164-Microscopy, Electron, Transmission, pubmed-meshheading:17625164-Molecular Sequence Data, pubmed-meshheading:17625164-Nucleic Acid Hybridization, pubmed-meshheading:17625164-Panax, pubmed-meshheading:17625164-Phylogeny, pubmed-meshheading:17625164-Polyamines, pubmed-meshheading:17625164-RNA, Bacterial, pubmed-meshheading:17625164-RNA, Ribosomal, 16S, pubmed-meshheading:17625164-Sequence Analysis, DNA, pubmed-meshheading:17625164-Sequence Homology, Nucleic Acid, pubmed-meshheading:17625164-Soil Microbiology, pubmed-meshheading:17625164-Vitamin K 2
pubmed:year
2007
pubmed:articleTitle
Chitinophaga ginsengisegetis sp. nov. and Chitinophaga ginsengisoli sp. nov., isolated from soil of a ginseng field in South Korea.
pubmed:affiliation
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't