Linked Life Data

17621362

Source:http://linkedlifedata.com/resource/pubmed/id/17621362

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2007-7-16
pubmed:abstractText
Since October 2000, Iran has been declared polio-free by the World Health Organization (WHO). Despite the fact that poliomyelitis caused by polioviruses has been eliminated from Iran, the number of acute flaccid paralysis (AFP) cases has not been reduced. Therefore, it is of great importance to investigate the other viral agents that may cause AFP (mainly nonpolio enteroviruses, which play a significant role in the etiology of neurological syndromes). Some enteroviruses do not grow in the conventional cell lines that are being used for enterovirus detection. Furthermore, the virus titer is an important factor in the sensitivity of cell culture to detect the virus. The fact that cell culture is a time-consuming procedure is another reason to find a more practical method for enterovirus detection. Therefore, a more sensitive and rapid method should be used to detect enteroviruses as efficiently as possible in the stool specimens of AFP cases. The aim of this study was to evaluate cell culture and RT-PCR in enterovirus detection. Findings have shown that RT-PCR can increase the rate of nonpolio enterovirus detection by up to 10% in comparison with cell culture. Also, the rapid detection of enteroviruses by RT-PCR can decrease both the unnecessary use of antibiotics and the costs in clinical practice. For this reason, we find that RT-PCR is a more practical technique for enterovirus detection.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0887-8013
pubmed:author
pubmed:copyrightInfo
Copyright (c) 2007 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
232-6
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Comparison of cell culture with RT-PCR for enterovirus detection in stool specimens from patients with acute flaccid paralysis.
pubmed:affiliation
School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences, Tehran, Iran. zshoja@razi.tums.ac.ir
pubmed:publicationType
Journal Article, Comparative Study