Linked Life Data

17620013

Source:http://linkedlifedata.com/resource/pubmed/id/17620013

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
30
pubmed:dateCreated
2007-7-24
pubmed:abstractText
The portal vertex structure of the phage P22 is a 2.8 MDa molecular machine that mediates attachment and injection of the viral genome into the host Salmonella enterica serovar Typhimurium. Five proteins form this molecular machine: the portal protein, gp1; the tail-spike, gp9; the tail-needle, gp26, and the tail accessory factors, gp4 and gp10. In order to understand the assembly of the portal vertex structure, we have isolated the gene encoding tail accessory factor gp10 and defined its structural composition and assembly within the portal vertex structure. In solution, monomeric gp10 is a beta-sheet-rich protein with a stable conformational structure, which spontaneously assembles into hexamers, likely via a dimeric intermediate. This oligomerization enhances the structural stability of the protein, which then becomes competent for assembly to a preformed portal protein:gp4 complex, and acts as a structural adaptor bridging the nascent phage tail to gp26 and gp9. Notably, in vitro purified tail accessory factors gp4, gp10, and gp26 do not significantly interact with each other in solution, but their assembly takes place efficiently when these factors are added sequentially onto an immobilized portal protein. This suggests that the assembly of the P22 tail is a highly sequential and cooperative process, likely mediated by structural rearrangements in the assembly components. The assembled portal vertex structure represents both a membrane-binding and penetrating device as well as a plug that retains the pressurized phage DNA inside the capsid.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
31
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8776-84
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Role of gene 10 protein in the hierarchical assembly of the bacteriophage P22 portal vertex structure.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 E. Adams Street, Syracuse, New York 13210, USA.
pubmed:publicationType
Journal Article