Source:http://linkedlifedata.com/resource/pubmed/id/17613522
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
35
|
pubmed:dateCreated |
2007-8-27
|
pubmed:abstractText |
The molecular features that allow activation-induced cytidine deaminase (AID) to target Ig and certain non-Ig genes are not understood, although transcription has been implicated as one important parameter. We explored this issue by testing the mutability of a non-Ig transcription cassette in Ig and non-Ig loci of the chicken B cell line DT40. The cassette did not act as a stable long term mutation target but was able to be mutated in an AID-dependent manner for a limited time post-integration. This indicates that newly integrated DNA has molecular characteristics that render it susceptible to modification by AID, with implications for how targeting and mis-targeting of AID occurs.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Aug
|
pubmed:issn |
0021-9258
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
31
|
pubmed:volume |
282
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
25308-13
|
pubmed:dateRevised |
2007-12-3
|
pubmed:meshHeading |
pubmed-meshheading:17613522-Animals,
pubmed-meshheading:17613522-B-Lymphocytes,
pubmed-meshheading:17613522-Cell Line,
pubmed-meshheading:17613522-Chickens,
pubmed-meshheading:17613522-Cytidine Deaminase,
pubmed-meshheading:17613522-Humans,
pubmed-meshheading:17613522-Immunoglobulins,
pubmed-meshheading:17613522-Mutagenesis, Insertional,
pubmed-meshheading:17613522-Mutation,
pubmed-meshheading:17613522-Substrate Specificity,
pubmed-meshheading:17613522-Time Factors,
pubmed-meshheading:17613522-Transcription, Genetic
|
pubmed:year |
2007
|
pubmed:articleTitle |
Activation-induced cytidine deaminase-mediated sequence diversification is transiently targeted to newly integrated DNA substrates.
|
pubmed:affiliation |
Department of Immunobiology, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06520-8011, USA.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|