Linked Life Data

17612296

Source:http://linkedlifedata.com/resource/pubmed/id/17612296

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2007-7-6
pubmed:abstractText
Aggregate culture provides a three-dimensional (3-D) environment for differentiating or differentiated cells; it is particularly useful to study in vitro chondrogenesis and cartilage biology. We have recently ported this method from a conical tube-based format to a 96-well plate format for the study of mesenchymal stem cell (MSC) chondrogenesis. The microplate format has greatly reduced the workload and materials cost, while maintaining reproducible chondrogenic differentiation. A long-term goal is to fully automate aggregate culture--this requires critically identifying all the indispensable steps of the protocol. Robotic laboratory equipment for manipulating microplate assays are commercially available; however centrifugation steps are difficult to implement automatically. We, therefore, tested whether the centrifugation step can be eliminated, thus significantly streamlining the assay workflow. By comparing aggregates prepared from human bone marrow-derived MSCs (hMSCs) that were formed either through centrifugation or through free sedimentation, we found that both methods produce aggregates with similar formation kinetics, and that there was no perceptible difference in the timing of the appearance of markers of chondrogenesis. Thus, it appears safe to eliminate the centrifugation step from the aggregate culture protocol. This results in significant time and effort savings and paves the way for future full automation of the aggregate assay.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17612296-10951362, http://linkedlifedata.com/resource/pubmed/commentcorrection/17612296-11241668, http://linkedlifedata.com/resource/pubmed/commentcorrection/17612296-15280587, http://linkedlifedata.com/resource/pubmed/commentcorrection/17612296-1581112, http://linkedlifedata.com/resource/pubmed/commentcorrection/17612296-16312217, http://linkedlifedata.com/resource/pubmed/commentcorrection/17612296-16590779, http://linkedlifedata.com/resource/pubmed/commentcorrection/17612296-1772427, http://linkedlifedata.com/resource/pubmed/commentcorrection/17612296-3200840, http://linkedlifedata.com/resource/pubmed/commentcorrection/17612296-8063865, http://linkedlifedata.com/resource/pubmed/commentcorrection/17612296-8344460, http://linkedlifedata.com/resource/pubmed/commentcorrection/17612296-9457080, http://linkedlifedata.com/resource/pubmed/commentcorrection/17612296-9875932
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0736-6205
pubmed:author
pubmed:issnType
Print
pubmed:volume
42
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
732, 734-7
pubmed:dateRevised
2011-7-25
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Simplification of aggregate culture of human mesenchymal stem cells as a chondrogenic screening assay.
pubmed:affiliation
Skeletal Research Center, Department of Biology, Case Western Reserve University, Cleveland, OH 44106, USA. Jean.Welter@case.edu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Technical Report, Research Support, N.I.H., Extramural