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rdf:type |
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lifeskim:mentions |
umls-concept:C0017968,
umls-concept:C0020860,
umls-concept:C0021311,
umls-concept:C0030705,
umls-concept:C0040896,
umls-concept:C0205369,
umls-concept:C0439234,
umls-concept:C0439851,
umls-concept:C0681840,
umls-concept:C0871261,
umls-concept:C1552596,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C1947931,
umls-concept:C2911692
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pubmed:issue |
9
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pubmed:dateCreated |
2007-8-22
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pubmed:abstractText |
The aim of this study was to evaluate the humoral immune response in late human trichinellosis with particular attention to the presence of IgG4 antibodies directed against the Trichinella-45-kDa glycoprotein (gp). This study re-evaluates subjects 15 years after they were involved in a trichinellosis outbreak that occurred in Central Italy following the consumption of raw boar meat infected with Trichinella britovi. The results show that ELISA tests using the E/S antigen identified five IgM- and eight IgG-positive patients and no IgA-positive patients. Tests using immunoblot (IB) with E/S antigens identified three IgM-, five IgA-, seven- IgG1- and three IgG4-positive sera. When the purified 45-kDa gp was used as an antigen, the IB revealed that six of the ten sera tested were positive for IgG4. Sera were also evaluated with a commercial kit, revealing that 11 of 12 patients had a highly sensitive reactivity against Trichinella proteins (64 and 44-43 kDa). In conclusion, humoral immune response against Trichinella is still present in these patients 15 years after the initial infection, including an IgG4 response directed to the 45-kDa gp.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0934-9723
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
641-5
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:17610096-Animals,
pubmed-meshheading:17610096-Antibodies, Helminth,
pubmed-meshheading:17610096-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:17610096-Follow-Up Studies,
pubmed-meshheading:17610096-Glycoproteins,
pubmed-meshheading:17610096-Helminth Proteins,
pubmed-meshheading:17610096-Humans,
pubmed-meshheading:17610096-Immunoblotting,
pubmed-meshheading:17610096-Immunoglobulin A,
pubmed-meshheading:17610096-Immunoglobulin G,
pubmed-meshheading:17610096-Immunoglobulin M,
pubmed-meshheading:17610096-Italy,
pubmed-meshheading:17610096-Trichinella,
pubmed-meshheading:17610096-Trichinellosis
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pubmed:year |
2007
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pubmed:articleTitle |
Specific IgG4 response directed against the 45-kDa glycoprotein in trichinellosis: a re-evaluation of patients 15 years after infection.
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pubmed:affiliation |
Department of Parasitology, Diagnostic Laboratory for Infectious Diseases and Perinatal Screening (LIS), National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands.
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pubmed:publicationType |
Journal Article
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