Linked Life Data

17606416

Source:http://linkedlifedata.com/resource/pubmed/id/17606416

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2007-8-31
pubmed:abstractText
A capillary zone electrophoresis method has been developed for the direct determination of norfloxacin in the physiological perfusate of isolated rat liver. Norfloxacin and the internal standard triamterene were detected using laser-induced fluorescence (LIF) detection with the excitation and emission wavelength of 325 and 435 nm, respectively. The background electrolyte (BGE) was 50 mM phosphate buffer (pH 4.6). The effect of pH and concentration of BGE on the electrophoretic migration and fluorescence response of analytes were examined. Calibration curves were linear over a wide range of 0.01-100 microg/mL. The limit of quantitation was 0.01 microg/mL. The intra- and inter-day relative standard deviation was 3.7%, or less, and the accuracy was 93.2% of the nominal concentration. No endogenous substances were found to interfere. The method was used to characterize the steady-state and transient pharmacokinetics of norfloxacin in the rat liver.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1570-0232
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
856
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
381-5
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Determination of norfloxacin in rat liver perfusate using capillary electrophoresis with laser-induced fluorescence detection.
pubmed:affiliation
Department of Pharmacy, Chia-Nan University of Pharmacy and Science, No. 60, Section 1, Erh-Jen Road, Jen-Te, Tainan 71710, Taiwan. hccl@mail.chna.edu.tw
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Validation Studies