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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
|
| pubmed:dateCreated |
1992-2-10
|
| pubmed:abstractText |
Human immunodeficiency virus (HIV types 1 and 2) replication is controlled by the interaction of viral-encoded regulatory proteins and host cellular proteins with the viral long terminal repeat (LTR). The presence of HIV-1 and HIV-2 trans-activator proteins, tat1 and tat2, respectively, greatly increases viral gene expression from their homologous LTRs. It is unclear if the cellular factors that support tat1-directed trans-activation of the HIV-1 LTR are the same for tat2 trans-activation of the HIV-2 LTR. Human-Chinese hamster ovary hybrid cell clones were used to probe for human chromosomes involved in regulating HIV-1 and HIV-2 tat-directed transactivation. DNA transfection experiments showed that the presence of human chromosome 12 in human-hamster hybrid clones was necessary for high-level tat-directed trans-activation of the HIV-1 and -2 LTR. Cross-trans-activation of the HIV-2 LTR by tat1 was found to be chromosome 12 independent. In addition, chromosome 12 did not support trans-activation of another human retrovirus (human T-cell leukemia virus type I). Our results suggest that HIV-1 and -2 have evolved to employ a cellular pathway(s) encoded on human chromosome 12 for supporting homologous tat-directed trans-activation. Trans-activation of the HIV-2 LTR by tat1 in chromosome 12-minus cells suggests that multiple cellular pathways can be recruited to trans-activate the HIV-2 LTR and that these pathways may have been important in an HIV-like progenitor virus.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0889-2229
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
7
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
877-82
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:1760228-Animals,
pubmed-meshheading:1760228-Base Sequence,
pubmed-meshheading:1760228-CHO Cells,
pubmed-meshheading:1760228-Chromosomes, Human, Pair 12,
pubmed-meshheading:1760228-Cricetinae,
pubmed-meshheading:1760228-Cricetulus,
pubmed-meshheading:1760228-Female,
pubmed-meshheading:1760228-HIV Long Terminal Repeat,
pubmed-meshheading:1760228-HIV-1,
pubmed-meshheading:1760228-HIV-2,
pubmed-meshheading:1760228-Humans,
pubmed-meshheading:1760228-Hybrid Cells,
pubmed-meshheading:1760228-Molecular Sequence Data,
pubmed-meshheading:1760228-Transcriptional Activation,
pubmed-meshheading:1760228-Transfection
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
Human chromosome-dependent and -independent pathways for HIV-2 trans-activation.
|
| pubmed:affiliation |
Centers for Disease Control, Atlanta, GA 30333.
|
| pubmed:publicationType |
Journal Article
|