Linked Life Data

17600970

Source:http://linkedlifedata.com/resource/pubmed/id/17600970

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2007-6-29
pubmed:abstractText
The transcription factor Pax5 is essential for B cell commitment and development. Although the detail Pax5 expression pattern within the hemopoietic system is still largely unknown, we previously reported that Pax5 is monoallelically transcribed in pro-B and mature B cells. In this study, we have investigated the expression of Pax5 at single-cell resolution by inserting a GFP or human Cd2 indicator gene under the translational control of an internal ribosomal entry sequence into the 3' untranslated region of Pax5. These insertions were noninvasive, as B cell development was normal in Pax5(ihCd2/ihCd2) and Pax5(ihGFP/iGFP) mice. Transheterozygous Pax5(ihCd2/iGFP) mice coexpressed GPF and human CD2 at similar levels from pro-B to mature B cells, thus demonstrating biallelic expression of Pax5 at all stages of B cell development. No reporter gene expression could be detected in plasma cells and non-B cells of hemopoietic system. Moreover, the vast majority of common lymphoid progenitors and pre-pro-B in the bone marrow of Pax5(ihGFP/iGFP) mice did not yet express GFP, indicating that Pax5 expression is fully switched on only during the transition form uncommitted pre-pro-B cells to committed pro-B cells. Hence, the transcriptional initiation and B cell-specific expression of Pax5 is entirely consistent with its B cell lineage commitment function.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
178
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8222-8
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Reporter gene insertions reveal a strictly B lymphoid-specific expression pattern of Pax5 in support of its B cell identity function.
pubmed:affiliation
Research Institute of Molecular Pathology, Vienna Biocenter, Austria.
pubmed:publicationType
Journal Article, Comment, Research Support, Non-U.S. Gov't