Linked Life Data

17597201

Source:http://linkedlifedata.com/resource/pubmed/id/17597201

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
28
pubmed:dateCreated
2007-7-19
pubmed:abstractText
Multi-functional bilayer polymeric coatings are prepared with both controlled nitric oxide (NO) release and surface-bound active thrombomodulin (TM) alone or in combination with immobilized heparin. The outer-layer is made of CarboSil, a commercially available copolymer of silicone rubber (SR) and polyurethane (PU). The CarboSil is either carboxylated or aminated via an allophanate reaction with a diisocyanate compound followed by a urea-forming reaction between the generated isocyanate group of the polymer and the amine group of an amino acid (glycine), an oligopeptide (triglycine) or a diamine. The carboxylated CarboSil can then be used to immobilize TM through the formation of an amide bond between the surface carboxylic acid groups and the lysine residues of TM. Aminated CarboSil can also be employed to initially couple heparin to the surface, and then the carboxylic acid groups on heparin can be further used to anchor TM. Both surface-bound TM and heparin's activity are evaluated by chromogenic assays and found to be at clinically significant levels. The underlying NO release layer is made with another commercial SR-PU copolymer (PurSil) mixed with a lipophilic NO donor (N-diazeniumdiolated dibutylhexanediamine (DBHD/N(2)O(2))). The NO release rate can be tuned by changing the thickness of top coatings, and the duration of NO release at physiologically relevant levels can be as long as 2 weeks. The combination of controlled NO release as well as immobilized active TM and heparin from/on the same polymeric surface mimics the highly thromboresistant endothelium layer. Hence, such multifunctional polymer coatings should provide more blood-compatible surfaces for biomedical devices.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17597201-11101159, http://linkedlifedata.com/resource/pubmed/commentcorrection/17597201-12563226, http://linkedlifedata.com/resource/pubmed/commentcorrection/17597201-12903997, http://linkedlifedata.com/resource/pubmed/commentcorrection/17597201-14613318, http://linkedlifedata.com/resource/pubmed/commentcorrection/17597201-15109834, http://linkedlifedata.com/resource/pubmed/commentcorrection/17597201-15941584, http://linkedlifedata.com/resource/pubmed/commentcorrection/17597201-16011323, http://linkedlifedata.com/resource/pubmed/commentcorrection/17597201-16368133, http://linkedlifedata.com/resource/pubmed/commentcorrection/17597201-17252544, http://linkedlifedata.com/resource/pubmed/commentcorrection/17597201-1852778, http://linkedlifedata.com/resource/pubmed/commentcorrection/17597201-2165069, http://linkedlifedata.com/resource/pubmed/commentcorrection/17597201-2538457, http://linkedlifedata.com/resource/pubmed/commentcorrection/17597201-3015228, http://linkedlifedata.com/resource/pubmed/commentcorrection/17597201-6715365, http://linkedlifedata.com/resource/pubmed/commentcorrection/17597201-6895633, http://linkedlifedata.com/resource/pubmed/commentcorrection/17597201-8134835, http://linkedlifedata.com/resource/pubmed/commentcorrection/17597201-8962952, http://linkedlifedata.com/resource/pubmed/commentcorrection/17597201-9751678
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0142-9612
pubmed:author
pubmed:issnType
Print
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4047-55
pubmed:dateRevised
2011-3-18
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Polymeric coatings that mimic the endothelium: combining nitric oxide release with surface-bound active thrombomodulin and heparin.
pubmed:affiliation
Department of Chemistry, The University of Michigan, 930 North University Avenue, Ann Arbor, MI 48109-1055, USA.
pubmed:publicationType
Journal Article, Evaluation Studies, Research Support, N.I.H., Extramural