Linked Life Data

17596369

Source:http://linkedlifedata.com/resource/pubmed/id/17596369

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2007-8-6
pubmed:abstractText
The VITEK 2 and Phoenix extended-spectrum beta-lactamase (ESBL) detection systems, which comprise confirmatory tests and expert systems, were evaluated for their ability to discriminate between 102 well-characterized strains of ESBL-positive or -negative Escherichia coli, Klebsiella pneumoniae, and Klebsiella oxytoca. At least 38 distinct ESBLs were included. The strains were chosen to include some known to cause false-positive and false-negative CLSI ESBL confirmatory test results. Therefore, enzyme characterizations, rather than CLSI tests, were the reference methods for the Phoenix and VITEK 2 evaluations. A third arm of the study was conducted with the Phoenix test using two normally inactive expert rules intended to enhance ESBL detection, in addition to using the currently available software. The Phoenix ESBL confirmatory test and unmodified expert system exhibited 96% sensitivity and 81% specificity for ESBL detection. Activation of the two additional rules increased sensitivity to 99% but reduced the specificity to 58%. The VITEK 2 ESBL confirmatory test exhibited 91% sensitivity, which was reduced to 89% sensitivity by its expert system, while its specificity was 85%. Many of the expert system interpretations of both instruments were helpful, but some were suboptimal. The VITEK 2 expert system was potentially more frustrating because it provided more inconclusive interpretations of the results. Considering the high degree of diagnostic difficulty posed by the strains, both ESBL confirmatory tests were highly sensitive. The expert systems of both instruments require modification to update and enhance their utility.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17596369-10348759, http://linkedlifedata.com/resource/pubmed/commentcorrection/17596369-11294735, http://linkedlifedata.com/resource/pubmed/commentcorrection/17596369-11427542, http://linkedlifedata.com/resource/pubmed/commentcorrection/17596369-12022255, http://linkedlifedata.com/resource/pubmed/commentcorrection/17596369-12354869, http://linkedlifedata.com/resource/pubmed/commentcorrection/17596369-12573548, http://linkedlifedata.com/resource/pubmed/commentcorrection/17596369-12615876, http://linkedlifedata.com/resource/pubmed/commentcorrection/17596369-12682131, http://linkedlifedata.com/resource/pubmed/commentcorrection/17596369-15206053, http://linkedlifedata.com/resource/pubmed/commentcorrection/17596369-15561858, http://linkedlifedata.com/resource/pubmed/commentcorrection/17596369-15673765, http://linkedlifedata.com/resource/pubmed/commentcorrection/17596369-15980389, http://linkedlifedata.com/resource/pubmed/commentcorrection/17596369-16223952, http://linkedlifedata.com/resource/pubmed/commentcorrection/17596369-16954257, http://linkedlifedata.com/resource/pubmed/commentcorrection/17596369-16954267, http://linkedlifedata.com/resource/pubmed/commentcorrection/17596369-17287329, http://linkedlifedata.com/resource/pubmed/commentcorrection/17596369-1952834
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
45
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2380-4
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Comparison of Phoenix and VITEK 2 extended-spectrum-beta-lactamase detection tests for analysis of Escherichia coli and Klebsiella isolates with well-characterized beta-lactamases.
pubmed:affiliation
Department of Medical Microbiology and Immunology, Creighton University School of Medicine, Omaha, Nebraska 68178, USA. kstaac@creighton.edu
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't, Evaluation Studies