|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
8
|
|
pubmed:dateCreated |
2007-7-19
|
|
pubmed:abstractText |
microRNAs (miRNAs) serve as post-transcriptional regulators of gene expression, by guiding effector complexes (miRNPs) to target RNAs. Although considerable progress has been made in computational methods to identify miRNA targets, only a relatively limited assessment of their ability to function in vivo has been reported. Here we describe an alternative approach to miRNA target identification based on a biochemical method for purifying miRNP complexes with associated miRNAs and bound mRNA targets. Microarray analysis revealed a high degree of enrichment for miRNA complementary sites in the 3'UTRs of the miRNP-associated mRNAs. mRNAs specifically associated with an individual miRNA were identified by comparing the miRNP-associated mRNAs from wild-type flies and mutant flies lacking miR-1, and their regulation by the miRNA was validated. This approach provides a means to identify functional miRNA targets based on their physical interaction in vivo.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-10504710,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-11318874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-11403571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-12154197,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-12679032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-14691535,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-14697198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-14709173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-14744438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-15014042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-15131085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-15143321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-15231716,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-15260970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-15620649,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-15652477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-15685193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-15723116,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-15738385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-15806104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-15809034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-16054033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-16081698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-16103902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-16141061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-16166373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-16224044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-16239240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-16289642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-16337999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-16458514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-16481470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-16484454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-16495412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-16581772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-16669754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-16736023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-16815998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-17096367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-17127242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-17200520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-6828386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17592038-8632795
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
1355-8382
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
13
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1198-204
|
|
pubmed:dateRevised |
2011-11-17
|
|
pubmed:meshHeading |
|
|
pubmed:year |
2007
|
|
pubmed:articleTitle |
Isolation of microRNA targets by miRNP immunopurification.
|
|
pubmed:affiliation |
European Molecular Biology Laboratory, Heidelberg, Germany.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
