Source:http://linkedlifedata.com/resource/pubmed/id/17584512
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2007-8-6
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pubmed:databankReference | |
pubmed:abstractText |
A novel lysozyme cDNA from the Pacific oyster, Crassostrea gigas, was identified. This second lysozyme from the Pacific oyster was designated as CGL-2. The complete CGL-2 cDNA sequence comprises of 536 bp, and 429 bp of the open reading frame encodes 147 bp of amino acid residues. Estimated CGL-2 molecular characteristics (isoelectric point and numbers of peptide recognition sites) resembled those of cv-lysozyme 2, a digestive lysozyme of the eastern oyster, Crassostrea virginica. Moreover, CGL-2 is phylogenetically homologous to the cv-lysozyme 2, indicating that CGL-2 and cv-lysozyme 2 evolved from the same ancestor protein for adaptation to the digestive environment. In situ hybridization revealed that the CGL-2 gene is expressed in digestive cells. It is noteworthy that the other Pacific oyster lysozyme, CGL-1, was also transcribed in the same cells. Presence and expression of multiple lysozymes in the digestive diverticula suggest that CGL-1 and CGL-2 might play complementary roles in digestive organs.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1096-4959
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
148
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
160-6
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pubmed:meshHeading |
pubmed-meshheading:17584512-Amino Acid Sequence,
pubmed-meshheading:17584512-Animals,
pubmed-meshheading:17584512-Base Sequence,
pubmed-meshheading:17584512-Cloning, Molecular,
pubmed-meshheading:17584512-Crassostrea,
pubmed-meshheading:17584512-DNA, Complementary,
pubmed-meshheading:17584512-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:17584512-In Situ Hybridization,
pubmed-meshheading:17584512-Molecular Sequence Data,
pubmed-meshheading:17584512-Muramidase,
pubmed-meshheading:17584512-Phylogeny,
pubmed-meshheading:17584512-Protein Transport,
pubmed-meshheading:17584512-Sequence Alignment,
pubmed-meshheading:17584512-Sequence Analysis, DNA
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pubmed:year |
2007
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pubmed:articleTitle |
cDNA cloning and in situ hybridization of a novel lysozyme in the Pacific oyster, Crassostrea gigas.
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pubmed:affiliation |
Aquacultural Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori Amamiya-machi, Aoba-ku, Sendai, Miyagi 981-8555, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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