| pubmed-article:17584167 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17584167 | lifeskim:mentions | umls-concept:C0041197 | lld:lifeskim |
| pubmed-article:17584167 | lifeskim:mentions | umls-concept:C0009017 | lld:lifeskim |
| pubmed-article:17584167 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:17584167 | lifeskim:mentions | umls-concept:C0323632 | lld:lifeskim |
| pubmed-article:17584167 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:17584167 | pubmed:dateCreated | 2007-6-22 | lld:pubmed |
| pubmed-article:17584167 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17584167 | pubmed:abstractText | Storage mites have been recognized as a cause of asthma and rhinitis. Studies from several countries have shown that the IgE-mediated allergy to storage mites is of considerable importance, especially in rural populations. This study aimed to identify and characterize new allergens from Tyrophagus putrescentiae. A partial cDNA sequence encoding tropomyosin was isolated from the cDNA library by immunoscreening using anti-mouse IgG1 sera raised against T. putrescentiae whole body extract. The deduced amino acid sequence shares 64-94% identity with previously known allergenic tropomyosins. Its recombinant protein was produced by using a pET 28b expression system and purified by affinity chromatography using Ni-NTA agarose. The IgE reactivities of tropomyosins from T. putrescentiae and Dermatophagoides farinae were compared by enzyme linked immunosorbent assay (ELISA). Recombinant Tyr p 10 showed 12.5% (5/40) IgE-binding reactivity, whereas recombinant Der f 10 showed 25% (10/40) IgE-binding reactivity against the same sera from storage mite-sensitized and house dust mite-sensitized subjects. Both recombinant Tyr p 10 and Der f 10 showed little inhibition of IgE binding to T. putrescentiae crude extract by ELISA. Tropomyosin seems to contribute only a small portion of the cross-reactivity with house dust mites. | lld:pubmed |
| pubmed-article:17584167 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17584167 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17584167 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17584167 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17584167 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17584167 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17584167 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17584167 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17584167 | pubmed:issn | 0929-8665 | lld:pubmed |
| pubmed-article:17584167 | pubmed:author | pubmed-author:SpilhausAA | lld:pubmed |
| pubmed-article:17584167 | pubmed:author | pubmed-author:YongTai-SoonT... | lld:pubmed |
| pubmed-article:17584167 | pubmed:author | pubmed-author:ReeHan-IlHI | lld:pubmed |
| pubmed-article:17584167 | pubmed:author | pubmed-author:LeeIn-YongIY | lld:pubmed |
| pubmed-article:17584167 | pubmed:author | pubmed-author:HongChein-Soo... | lld:pubmed |
| pubmed-article:17584167 | pubmed:author | pubmed-author:LeeJae SikJS | lld:pubmed |
| pubmed-article:17584167 | pubmed:author | pubmed-author:LeeJongweonJ | lld:pubmed |
| pubmed-article:17584167 | pubmed:author | pubmed-author:JeongKyoung... | lld:pubmed |
| pubmed-article:17584167 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17584167 | pubmed:volume | 14 | lld:pubmed |
| pubmed-article:17584167 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17584167 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17584167 | pubmed:pagination | 431-6 | lld:pubmed |
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| pubmed-article:17584167 | pubmed:meshHeading | pubmed-meshheading:17584167... | lld:pubmed |
| pubmed-article:17584167 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17584167 | pubmed:articleTitle | Molecular cloning and the allergenic characterization of tropomyosin from Tyrophagus putrescentiae. | lld:pubmed |
| pubmed-article:17584167 | pubmed:affiliation | Department of Parasitology and Institute of Tropical Medicine, Brain Korea 21 Project for Medical Science, Yonsei University, College of Medicine, Seoul, Korea. jeongky@paran.com | lld:pubmed |
| pubmed-article:17584167 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17584167 | lld:pubmed |
