Source:http://linkedlifedata.com/resource/pubmed/id/17584167
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2007-6-22
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| pubmed:databankReference | |
| pubmed:abstractText |
Storage mites have been recognized as a cause of asthma and rhinitis. Studies from several countries have shown that the IgE-mediated allergy to storage mites is of considerable importance, especially in rural populations. This study aimed to identify and characterize new allergens from Tyrophagus putrescentiae. A partial cDNA sequence encoding tropomyosin was isolated from the cDNA library by immunoscreening using anti-mouse IgG1 sera raised against T. putrescentiae whole body extract. The deduced amino acid sequence shares 64-94% identity with previously known allergenic tropomyosins. Its recombinant protein was produced by using a pET 28b expression system and purified by affinity chromatography using Ni-NTA agarose. The IgE reactivities of tropomyosins from T. putrescentiae and Dermatophagoides farinae were compared by enzyme linked immunosorbent assay (ELISA). Recombinant Tyr p 10 showed 12.5% (5/40) IgE-binding reactivity, whereas recombinant Der f 10 showed 25% (10/40) IgE-binding reactivity against the same sera from storage mite-sensitized and house dust mite-sensitized subjects. Both recombinant Tyr p 10 and Der f 10 showed little inhibition of IgE binding to T. putrescentiae crude extract by ELISA. Tropomyosin seems to contribute only a small portion of the cross-reactivity with house dust mites.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:issn |
0929-8665
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
14
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
431-6
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| pubmed:meshHeading |
pubmed-meshheading:17584167-Acaridae,
pubmed-meshheading:17584167-Adolescent,
pubmed-meshheading:17584167-Adult,
pubmed-meshheading:17584167-Aged,
pubmed-meshheading:17584167-Allergens,
pubmed-meshheading:17584167-Amino Acid Sequence,
pubmed-meshheading:17584167-Animals,
pubmed-meshheading:17584167-Child,
pubmed-meshheading:17584167-Cloning, Molecular,
pubmed-meshheading:17584167-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:17584167-Humans,
pubmed-meshheading:17584167-Immunoglobulin E,
pubmed-meshheading:17584167-Mice,
pubmed-meshheading:17584167-Middle Aged,
pubmed-meshheading:17584167-Molecular Sequence Data,
pubmed-meshheading:17584167-Recombinant Proteins,
pubmed-meshheading:17584167-Sequence Alignment,
pubmed-meshheading:17584167-Tropomyosin
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| pubmed:year |
2007
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| pubmed:articleTitle |
Molecular cloning and the allergenic characterization of tropomyosin from Tyrophagus putrescentiae.
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| pubmed:affiliation |
Department of Parasitology and Institute of Tropical Medicine, Brain Korea 21 Project for Medical Science, Yonsei University, College of Medicine, Seoul, Korea. jeongky@paran.com
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| pubmed:publicationType |
Journal Article
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