Linked Life Data

17583784

Source:http://linkedlifedata.com/resource/pubmed/id/17583784

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2007-7-30
pubmed:abstractText
Recently it has been demonstrated that, along with sperm, some of its RNA can be introduced into the oocyte during fertilization, which stays stable until the activation of the embryonic genome. Originally it was thought that RNA present in semen relates to contamination from somatic cells and/or immature sperm both containing substantially higher amounts of RNA than the fertilizing sperm. However, RNA is still found after stringent washing through density gradients resulting in a sperm fraction that is translational silenced and devoid of cytosolic rRNA and thus of potential RNA contamination-which is not transferable to the oocyte. Sperm only delivers a relatively small amount of paternal RNA (5-10 fg) into the fertilized oocyte when compared to the amount of maternal RNA (approximately 1 ng). Pooled human sperm contains about 5000 different mRNA sequences of which half are common between ejaculates. Besides mRNA sperm also contains small sperm RNA molecules that might interfere in gene expression (iRNA). In human sperm already more than 68 putative iRNAs have been identified and 15 of them may specifically inhibit genes that are only active during early embryonic development. The composition and quantity of sperm RNA is considered to be a valuable diagnostic tool for male fertility. However, only a subpopulation of the purified mature sperm fraction (with a yet unknown composition and quantity of RNA) will appropriately respond to capacitation media to become competent to fertilize the oocyte. In this review the origin and function of sperm borne RNA transferred into the oocyte is discussed along with their putative role in early embryogenesis, which still needs to be experimentally proven.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0093-691X
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
68 Suppl 1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
S147-55
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
A possible role for sperm RNA in early embryo development.
pubmed:affiliation
Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, The Netherlands.
pubmed:publicationType
Journal Article, Review, Research Support, Non-U.S. Gov't