Linked Life Data

17582838

Source:http://linkedlifedata.com/resource/pubmed/id/17582838

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2007-6-20
pubmed:abstractText
We have proposed modular tissue engineering as a strategy to construct vascularized tissues containing multiple cell types. To create a modular construct, instead of seeding a preformed scaffold, cells were encapsulated within sub-mm modules, and the outer surface of these modules was covered with a layer of endothelial cells. Modules were then added to a larger structure (here by filling a tube) to form the modular construct. Through a systematic process of materials selection, collagen, human umbilical vein endothelial cells (HUVECs), and HepG2 cells, a human hepatoma cell line, were identified as suitable components for module formation, at least for initial studies. A method, which involved cutting and shaping the modules within a tubular mold, was developed to fabricate sub-mm, cylindrical, collagen modules that contained viable, functioning HepG2 cells and that could be seeded with a surface layer of HUVECs. Module dimensions were reproducible and easily altered in a controlled fashion if desired. The module fabrication process developed here not only generated modules suitable for the assembly of a prototype modular construct, but also could potentially be used more generally for other applications for which the goal is to form submm-diameter cylinders from soft hydrogels.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1076-3279
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1069-78
pubmed:dateRevised
2007-12-3
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Design and fabrication of sub-mm-sized modules containing encapsulated cells for modular tissue engineering.
pubmed:affiliation
Department of Chemical Engineering and Applied Chemistry, Institute of Biomaterials and Biomedical Engineering, University of Toronto, Ontario, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural