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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2007-6-20
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pubmed:abstractText |
To develop a new fusion protein (RGD)3/tTF for the therapy of the selective thrombosis of tumor blood vessels. The fused gene (RGD) 3/tTF was reconstructed by PCR, was cloned into vector pET22 b(+), and expressed in E. coli BL21 (DE3). The fusion protein was purified through Nickel-affinity chromatography column. The tTF activity of the fusion protein was detected by clotting assay and F X activation assay. The specific binding of (RGD) 3/tTF to alphavbeta3 was analyzed by indirect ELISA. The recombinant plasmid pET22 b(+)/(RGD)3/tTF was obtained and expressed in E. coli BL21 (DE3). The purified fusion protein could induce blood coagulation, activiate F X. The ability of (RGD) 3/tTF binding specifically to alphavbeta3 was increased by 32%, compared with RGD/tTF. A new fusion protein (RGD) 3/tTF was successfully expressed in E. coli BL21 (DE3). The expressed proteins retained tTF activity and showed a higher binding to alphavbeta3 than that of RGD/tTF.
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pubmed:language |
chi
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1000-3061
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
409-12
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pubmed:meshHeading |
pubmed-meshheading:17577984-Animals,
pubmed-meshheading:17577984-Blood Coagulation,
pubmed-meshheading:17577984-Chromatography, Affinity,
pubmed-meshheading:17577984-Dose-Response Relationship, Drug,
pubmed-meshheading:17577984-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:17577984-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:17577984-Escherichia coli,
pubmed-meshheading:17577984-Factor Xa,
pubmed-meshheading:17577984-Gene Expression,
pubmed-meshheading:17577984-Humans,
pubmed-meshheading:17577984-Integrin alphaVbeta3,
pubmed-meshheading:17577984-Mice,
pubmed-meshheading:17577984-Oligopeptides,
pubmed-meshheading:17577984-Polymerase Chain Reaction,
pubmed-meshheading:17577984-Protein Binding,
pubmed-meshheading:17577984-Recombinant Fusion Proteins,
pubmed-meshheading:17577984-Thromboplastin
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pubmed:year |
2007
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pubmed:articleTitle |
[Gene expression and activities analysis of a new fusion protein (RGD) 3/tTF].
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pubmed:affiliation |
Cancer Research Center of Medical School, Xiamen University, Xiamen 361005, China. jhyan@xmu.edu.cn
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pubmed:publicationType |
Journal Article,
English Abstract
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