Linked Life Data

17576046

Source:http://linkedlifedata.com/resource/pubmed/id/17576046

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2007-7-30
pubmed:abstractText
The concentrations of extracellularly released nucleotides are controlled by metabolism via ecto-nucleotidases, but the precise physiological roles of the ecto-nucleoside triphosphate diphosphohydrolases in the modulation of purinergic receptor signalling are still unclear. Bacterial endotoxin lipopolysaccharide (LPS) treatment (administered intraperitoneally, 2 mg/kg body weight) of rats resulted in no significant changes in the overall ecto-nucleotidase activities of the hippocampus, however, LPS treatment did cause transient changes in the morphology of endothelial cells and pericytes and in the localization pattern of ecto-ATPase activity in rat hippocampus. The transient decrease in NTPDase1 (ecto-nucleoside triphosphate diphosphohydrolase1) activity, located on the luminal side of the endothelial cells, was balanced by increases in ecto-nucleotidase activities in pericytes and at other sites, consistent with an unchanged overall ecto-ATPase activity of the hippocampus. Since the transient loss of NTPDase1 activity was not accompanied by a loss of NTPDase1 protein, we hypothesize that LPS caused transient alterations in the lipid membranes, since NTPDase1 activity is known to be sensitive to changes in membrane structure via its transmembrane domains. After 2-3 days, the LPS-induced changes in cell morphology and ecto-nucleotidase localization disappeared. We conclude that a low dose of LPS causes transient changes in the localization pattern of ecto-nucleotidases in endothelial cells and pericytes, which, coupled with the observed cellular morphological changes, may indicate modified cellular signalling in the hippocampus.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0736-5748
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
275-82
pubmed:dateRevised
2010-4-30
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Transient changes in the localization and activity of ecto-nucleotidases in rat hippocampus following lipopolysaccharide treatment.
pubmed:affiliation
Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary. kittel@koki.hu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural