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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
29
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pubmed:dateCreated |
2007-7-2
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pubmed:abstractText |
One strategy to generate T-cell responses to tumors is to alter subdominant epitopes through substitution of amino acids that are optimal anchors for specific MHC molecules, termed heteroclitic epitopes. This approach is manually error-prone and time-consuming. In here, we describe a computer-based algorithm (EpitOptimizer) for the streamlined design of heteroclitic epitopes. Analysis of two cancer-related proteins showed that EpitOptimizer-generated peptides have enhanced MHC-I binding compared with their wild-type counterparts; and were able to induce stronger CD8+ T-cell responses against their native epitope. These data demonstrate that this approach can serve as the basis of epitope-engineering against cancer and intracellular pathogens.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0264-410X
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pubmed:author |
pubmed-author:EngelhornManuel EME,
pubmed-author:GregorPollyP,
pubmed-author:Guevara-PatiñoJosé AJA,
pubmed-author:HoughtonAlan NAN,
pubmed-author:HoughtonColin S BCS,
pubmed-author:LiuCailanC,
pubmed-author:LivingstonPhillip OPO,
pubmed-author:McCrackenJamesJ,
pubmed-author:OrlandiFrancescaF,
pubmed-author:SongDaD,
pubmed-author:WolchokJedd DJD
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5330-42
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pubmed:dateRevised |
2007-12-3
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pubmed:meshHeading |
pubmed-meshheading:17570567-Algorithms,
pubmed-meshheading:17570567-Amino Acid Sequence,
pubmed-meshheading:17570567-Animals,
pubmed-meshheading:17570567-CD8-Positive T-Lymphocytes,
pubmed-meshheading:17570567-Cancer Vaccines,
pubmed-meshheading:17570567-Computational Biology,
pubmed-meshheading:17570567-Cross Reactions,
pubmed-meshheading:17570567-Epitopes,
pubmed-meshheading:17570567-Female,
pubmed-meshheading:17570567-Histocompatibility Antigens Class I,
pubmed-meshheading:17570567-Mice,
pubmed-meshheading:17570567-Mice, Inbred C57BL,
pubmed-meshheading:17570567-Mice, Transgenic,
pubmed-meshheading:17570567-Models, Animal,
pubmed-meshheading:17570567-Molecular Sequence Data,
pubmed-meshheading:17570567-Mutagenesis, Site-Directed,
pubmed-meshheading:17570567-Neoplasms,
pubmed-meshheading:17570567-Peptides,
pubmed-meshheading:17570567-Protein Engineering
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pubmed:year |
2007
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pubmed:articleTitle |
Immunological validation of the EpitOptimizer program for streamlined design of heteroclitic epitopes.
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pubmed:affiliation |
The Swim Across America Laboratory, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, United States.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural,
Validation Studies
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