|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:dateCreated |
2007-7-2
|
|
pubmed:abstractText |
We have evaluated a sensitive screening assay for Mycobacterium tuberculosis (MTB) complex organisms and a specific assay for detecting Mycobacterium bovis DNA in lymph nodes taken from cattle with evidence of bovine tuberculosis. Underlying these series of experiments was the need for a versatile DNA extraction protocol which could handle tissue samples and with the potential for automation.The target for the screening assay was the multi-copy insertion element IS1081, present in 6 copies in the MTB complex. For confirmation of M. bovis we used primers flanking a specific deletion in the genome of M. bovis known as region of difference 4 (RD4). The sensitivity and specificity of these PCRs has been tested on genomic DNA from MTB complex reference strains, mycobacteria other than tuberculosis (MOTT), spiked samples and on clinical material.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-10320585,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-10619610,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-10793782,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-10808091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-11124091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-11283040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-11288522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-11409586,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-11463223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-11463237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-11562707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-11724883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-11891304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-12089245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-12661717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-12701839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-12788972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-12801063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-14709001,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-14715798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-15109433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-15872286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-15917808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-16242724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-16384663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-16434219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-16543179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-1691208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-328766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-6398578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-7496524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-7574484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-7579326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-7740761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-8664595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-8727904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-8812720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-9157152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17567891-9465157
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:issn |
1746-6148
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:volume |
3
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
12
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:17567891-Animals,
pubmed-meshheading:17567891-Bacteriological Techniques,
pubmed-meshheading:17567891-Cattle,
pubmed-meshheading:17567891-DNA, Bacterial,
pubmed-meshheading:17567891-DNA Primers,
pubmed-meshheading:17567891-DNA Transposable Elements,
pubmed-meshheading:17567891-Lymph Nodes,
pubmed-meshheading:17567891-Mycobacterium bovis,
pubmed-meshheading:17567891-Polymerase Chain Reaction,
pubmed-meshheading:17567891-Reproducibility of Results,
pubmed-meshheading:17567891-Sensitivity and Specificity,
pubmed-meshheading:17567891-Tuberculosis, Bovine
|
|
pubmed:year |
2007
|
|
pubmed:articleTitle |
Rapid detection of Mycobacterium bovis DNA in cattle lymph nodes with visible lesions using PCR.
|
|
pubmed:affiliation |
Centre for Microbiology and Infectious Diseases, Flowers Building, Imperial College, London, UK. gm.taylor@imperial.ac.uk
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
