Linked Life Data

1756781

Source:http://linkedlifedata.com/resource/pubmed/id/1756781

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1992-2-3
pubmed:abstractText
Published attempts to extract lipofuscin from crustaceans and fish to assess age for fisheries research purposes have used essentially the same extraction methodology applied to insects, but have neither shown a conclusive age-dependence of spectrally similar fluorescence nor proved its association with lipofuscin. The reported lipofuscin solvent extraction method for fleshflies, Sarcophaga bullata, was manipulated by varying wash volume. This revealed that almost all age-dependent blue fluorescent material persisting in lipid fractions was actually pteridine-like. This finding was consistent with some previous independent results for Musca domestica. Examination of reported lipofuscin extraction protocols for other insects suggested that this problem was probably widespread. The pteridines are known to occur in unusually high amounts in insects, accumulating with age specifically in some members of this group by storage excretion, probably as a terrestrial water conservation strategy. In addition, there is growing evidence in the gerontological literature for other groups that solvent extracted blue fluorescence is not a true measure of lipofuscin content in tissues. These findings provide considerable insight into anomalies in putative lipofuscin fluorescence data between the insects and various aquatic species and suggest that there may be little basis for expectations of age-dependent fluorescence from aquatic species when the same gross extraction and crude purification methods are used.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0531-5565
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
495-509
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
An alternative explanation for anomalies in "soluble lipofuscin" fluorescence data from insects, crustaceans, and other aquatic species.
pubmed:affiliation
Department of Zoology, University of Queensland, Australia.
pubmed:publicationType
Journal Article