Linked Life Data

17566275

Source:http://linkedlifedata.com/resource/pubmed/id/17566275

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2007-6-14
pubmed:abstractText
The zona pellucida that surrounds the mammalian oocyte plays a role in species-selective sperm-egg interactions. In the pig and cattle, the zona pellucida consists of ZPA, ZPB and ZPC. Sperm binding activity of porcine zona glycoproteins is conferred by tri- and tetra-antennary complex-type in cattle it is conferred by a high-mannose-type chain o f f ive mannose residues. Non-reducing terminal residues of these N-linked chains, beta-galactosyl residues in pig and alpha-mannosyl residues in cattle, are involved in the binding of zona glycoproteins to respective spermatozoa. The major N-linked chains of recombinant porcine ZPB expressed using the baculovirus-Sf9 cell expression system are pauci- and high-mannose-type chains that are different in structure to the major neutral N-linked chains of the porcine zona but similar to those of the bovine zona. The mixture of porcine ZPB/ZPC co-expressed in Sf9 cells binds to bovine sperm but not to porcine sperm, indicating an essential role of the N-linked chains in species-selective recognition of sperm in pig and cattle. Asn to Asp mutations at either of two of the N-glycosylation sites of ZPB, residue 203 or 220, significantly reduce the sperm-binding activity of the ZPB/ZPC mixture, while a similar mutation at Asn333 has no effect on binding. These results coincide with our previous report that tri- and tetra-antennary complex-type chains are localized at Asn220 in native porcine ZPB and suggest that the N-glycans located in the N-terminal half of the ZP domain of porcine ZPB are involved in sperm-zona binding.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:author
pubmed:volume
63
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
217-28
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Structural significance of N-glycans of the zona pellucida on species-selective recognition of spermatozoa between pig and cattle.
pubmed:affiliation
Graduate School of Science and Technology, Chiba University, 1-33, Yayoi-cho, Inage-ku, Chiba 263-8522, Japan. nyoneza@faculty.chiba-u.jp
pubmed:publicationType
Journal Article, Review