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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
|
pubmed:dateCreated |
1992-2-5
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pubmed:abstractText |
After removal, by high-salt extraction, of the loosely-bound components present in human placenta chromatin, tightly-bound cationic proteins could be solubilized, by acid extraction, from the 'stripped' chromatin, as well as from the 'stripped' loops or from the 'digested matrix'. These acid-soluble tightly-bound proteins are, in terms of apparent molecular mass and immunoreactivity, quite similar to the 'typical', loosely-bound histones, and, similarly to their 'loosely-bound' counterparts, they can be subdivided in distinct H1-, H2A-, H2B-, H3- and H4-like components, the 'digested matrix' being however characterized by the absence of tightly-bound H1. These tightly-bound histones, at variance from the 'typical' ones, readily find a right-handed helical conformation upon renaturation by progressive dialyses. The H1 components strongly differ also in their effects on enzymic DNA methylation: while 'typical' H1 has a strong inhibitory effect, its tightly-bound counterpart exerts a slight but definite stimulation.
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pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Dec
|
pubmed:issn |
0006-3002
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
2
|
pubmed:volume |
1129
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
|
pubmed:pagination |
43-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1756179-Chromatin,
pubmed-meshheading:1756179-Circular Dichroism,
pubmed-meshheading:1756179-DNA Modification Methylases,
pubmed-meshheading:1756179-Histones,
pubmed-meshheading:1756179-Humans,
pubmed-meshheading:1756179-Methylation,
pubmed-meshheading:1756179-Nucleic Acid Conformation,
pubmed-meshheading:1756179-Spectrophotometry, Ultraviolet
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pubmed:year |
1991
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pubmed:articleTitle |
Histones and DNA methylation in mammalian chromatin. II. Presence of non-inhibitory tightly-bound histones.
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pubmed:affiliation |
Department of Biochemical Sciences, University of Rome La Sapienza, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|