Linked Life Data

17556810

Source:http://linkedlifedata.com/resource/pubmed/id/17556810

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2007-6-8
pubmed:abstractText
A number of signal pathways have been found through which abundant calcium-stimulated protein kinase activity in plant is associated with calcium-dependent protein kinases (CDPKs) which act as the calcium sensors mediating numerous responses, including hormone signaling. Basing on previous studies, we made additional functional analysis of the gene AtCPK30 encoding a protein kinase in Arabidopsis. Results of semi-quantitative reverse transcription PCR (RT-PCR) analysis indicated that AtCPK30 was highly expressed in root and induced by ABA, IAA, 2,4-D, GA(3) and 6-BA treatment. The physiological roles of AtCPK30 were studied using a gain-of-function approach. Seedlings of AtCPK30 transgenic lines had longer primary roots than those plants of wild-type at the early stages. Interestingly, when these plants grew on MS lack of Ca(2+) including wild-type and transgenic lines, the roots of transgenic line were more sensitive to calcium, lack of Ca(2+) had less effect on roots of transgenic lines than those of wild-type. Treated with several plant hormones, such as ABA, IAA, GA(3) and 6-BA, the roots of seedlings of transgenic line developed abnormally because they were more sensitive to hormones. Furthermore, NPA relatively less inhibited emergency of lateral roots of transgenic line than those of the wild-type. Green fluorescent protein-CPK30 (GFP-CPK30) fusion protein studies revealed the localization of AtCPK30 to both cell wall and plasma membrane. These results suggest that AtCPK30 acts as the calcium sensor and involved in the hormone-signaling pathways.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1671-3877
pubmed:author
pubmed:issnType
Print
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
227-34
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
A calcium-dependent protein kinase is involved in plant hormone signal transduction in Arabidopsis.
pubmed:affiliation
Bioenergy and Biomaterial Research Center, Institute of Life Science and Biotechnology, Hunan University, Changsha 410082, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't