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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5830
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pubmed:dateCreated |
2007-6-8
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pubmed:abstractText |
We describe a sensitive mRNA profiling technology, PMAGE (for "polony multiplex analysis of gene expression"), which detects messenger RNAs (mRNAs) as rare as one transcript per three cells. PMAGE incorporates an improved ligation-based method to sequence 14-nucleotide tags derived from individual mRNA molecules. One sequence tag from each mRNA molecule is amplified onto a separate 1-micrometer bead, denoted as a polymerase colony or polony, and about 5 million polonies are arrayed in a flow cell for parallel sequencing. Using PMAGE, we identified early transcriptional changes that preceded pathological manifestations of hypertrophic cardiomyopathy in mice carrying a disease-causing mutation. PMAGE provided a comprehensive profile of cardiac mRNAs, including low-abundance mRNAs encoding signaling molecules and transcription factors that are likely to participate in disease pathogenesis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1095-9203
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
8
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pubmed:volume |
316
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1481-4
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pubmed:meshHeading |
pubmed-meshheading:17556586-Animals,
pubmed-meshheading:17556586-Cardiomyopathy, Hypertrophic,
pubmed-meshheading:17556586-DNA, Complementary,
pubmed-meshheading:17556586-Fibrosis,
pubmed-meshheading:17556586-Gene Expression Profiling,
pubmed-meshheading:17556586-Gene Expression Regulation,
pubmed-meshheading:17556586-Gene Library,
pubmed-meshheading:17556586-Heart Ventricles,
pubmed-meshheading:17556586-Mice,
pubmed-meshheading:17556586-Mutation,
pubmed-meshheading:17556586-Myocardial Contraction,
pubmed-meshheading:17556586-Myocardium,
pubmed-meshheading:17556586-Myosin Heavy Chains,
pubmed-meshheading:17556586-RNA, Messenger,
pubmed-meshheading:17556586-Reproducibility of Results,
pubmed-meshheading:17556586-Sensitivity and Specificity,
pubmed-meshheading:17556586-Sequence Analysis, DNA,
pubmed-meshheading:17556586-Templates, Genetic,
pubmed-meshheading:17556586-Transcription, Genetic,
pubmed-meshheading:17556586-Transcription Factors,
pubmed-meshheading:17556586-Ventricular Myosins
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pubmed:year |
2007
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pubmed:articleTitle |
Polony multiplex analysis of gene expression (PMAGE) in mouse hypertrophic cardiomyopathy.
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pubmed:affiliation |
Cardiovascular Division, Brigham and Women's Hospital, Boston, MA 02115, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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