Linked Life Data

17552899

Source:http://linkedlifedata.com/resource/pubmed/id/17552899

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-1-16
pubmed:abstractText
Mechanical properties of scaffolds seeded with mesenchymal stem cells used for cartilage repair seem to be one of the critical factors in possible joint resurfacing. In this paper, the effect of adding hyaluronic acid, hydroxyapatite nanoparticles or chitosan nanofibers into the cross-linked collagen I on the mechanical response of the lyophilized porous scaffold has been investigated in the dry state at 37 oC under tensile loading. Statistical significance of the results was evaluated using ANOVA analysis. The results showed that the addition of hyaluronic acid significantly (p<<0.05) reduced the tensile elastic modulus and enhanced the strength and deformation to failure of the modified cross-linked collagen I under the used test conditions. On the other hand, addition of hydroxyapatite nanoparticles and chitosan nanofibers, respectively, increased the elastic modulus of the modified collagen ten-fold and four-fold, respectively. Hydroxyapatite caused significant reduction in the ultimate deformation at break while chitosan nanofibers enhanced the ultimate deformation under tensile loading substantially (p<<0.05). The ultimate tensile deformation was significantly (p<<0.05) increased by addition of the chitosan nanofibers. The enhanced elastic modulus of the scaffold was translated into enhanced resistance of the porous scaffolds against mechanical load compared to scaffolds based on cross-linked neat collagen or collagen with hyaluronic acid with similar porosity. It can be concluded that enhancing the rigidity of the compact scaffold material by adding rigid chitosan nanofibers can improve the resistance of the porous scaffolds against compressive loading, which can provide more structural protection to the seeded mesenchymal stem cells when the construct is implanted into a lesion. Moreover, scaffolds with chitosan nanofibers seemed to enhance cell growth compared to the neat collagen I when tested in vitro as well as the scaffold stability, extending its resorption to more than 10 weeks.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0862-8408
pubmed:author
pubmed:issnType
Print
pubmed:volume
56 Suppl 1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
S17-25
pubmed:meshHeading
pubmed-meshheading:17552899-Animals, pubmed-meshheading:17552899-Biocompatible Materials, pubmed-meshheading:17552899-Cartilage, pubmed-meshheading:17552899-Cattle, pubmed-meshheading:17552899-Cell Proliferation, pubmed-meshheading:17552899-Cells, Cultured, pubmed-meshheading:17552899-Chitosan, pubmed-meshheading:17552899-Collagen Type I, pubmed-meshheading:17552899-Durapatite, pubmed-meshheading:17552899-Elasticity, pubmed-meshheading:17552899-Freeze Drying, pubmed-meshheading:17552899-Humans, pubmed-meshheading:17552899-Hyaluronic Acid, pubmed-meshheading:17552899-Materials Testing, pubmed-meshheading:17552899-Mesenchymal Stem Cells, pubmed-meshheading:17552899-Nanoparticles, pubmed-meshheading:17552899-Porosity, pubmed-meshheading:17552899-Prosthesis Failure, pubmed-meshheading:17552899-Tensile Strength, pubmed-meshheading:17552899-Tissue Engineering, pubmed-meshheading:17552899-Tissue Scaffolds
pubmed:year
2007
pubmed:articleTitle
Mechanical response of porous scaffolds for cartilage engineering.
pubmed:affiliation
Institute of Materials Chemistry, University of Technology, Brno, Czech Republic. jancar@fch.vutbr.cz
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't