Source:http://linkedlifedata.com/resource/pubmed/id/17549679
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
2007-9-13
|
| pubmed:abstractText |
A sensitive and selective high-performance liquid chromatography method was developed and validated to determine the prototype of salvianolic acid B and the metabolites of phenolic acids (protocatechuic acid, vanillic acid and ferulic acid) in rat tissues after oral administration of total phenolic acids and salvianolic acid B extracted from the roots of Salvia miltiorrhiza, respectively. The tissue samples were treated with a simple liquid-liquid extraction prior to HPLC. Analysis of the extract was performed on a reverse-phase C(18) column with a mobile phase consisting of acetonitrile and 0.05% trifluoracetic acid. The calibration curves for the four phenolic acids were linear in the given concentration ranges. The intra-day and inter-day relative standard deviations in the measurement of quality control samples were less than 10% and the accuracies were in the range of 88-115%. The average recoveries of all the tissues ranged from 78.0 to 111.8%. This method was successfully applied to evaluate the distribution of the four phenolic acids in rat tissues after oral administration of total phenolic acids of Salvia miltiorrhiza or salvianolic acid B and the possible metabolic pathway was illustrated.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Benzofurans,
http://linkedlifedata.com/resource/pubmed/chemical/Coumaric Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Drugs, Chinese Herbal,
http://linkedlifedata.com/resource/pubmed/chemical/Hydroxybenzoic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/ferulic acid,
http://linkedlifedata.com/resource/pubmed/chemical/phenolic acid,
http://linkedlifedata.com/resource/pubmed/chemical/salvianolic acid B
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0269-3879
|
| pubmed:author | |
| pubmed:copyrightInfo |
2007 John Wiley & Sons, Ltd
|
| pubmed:issnType |
Print
|
| pubmed:volume |
21
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1052-63
|
| pubmed:dateRevised |
2010-11-18
|
| pubmed:meshHeading |
pubmed-meshheading:17549679-Administration, Oral,
pubmed-meshheading:17549679-Animals,
pubmed-meshheading:17549679-Benzofurans,
pubmed-meshheading:17549679-Chemical Fractionation,
pubmed-meshheading:17549679-Chromatography, High Pressure Liquid,
pubmed-meshheading:17549679-Coumaric Acids,
pubmed-meshheading:17549679-Drugs, Chinese Herbal,
pubmed-meshheading:17549679-Hydrophobic and Hydrophilic Interactions,
pubmed-meshheading:17549679-Hydroxybenzoic Acids,
pubmed-meshheading:17549679-Male,
pubmed-meshheading:17549679-Metabolic Networks and Pathways,
pubmed-meshheading:17549679-Plant Roots,
pubmed-meshheading:17549679-Rats,
pubmed-meshheading:17549679-Rats, Sprague-Dawley,
pubmed-meshheading:17549679-Reference Standards,
pubmed-meshheading:17549679-Salvia miltiorrhiza,
pubmed-meshheading:17549679-Specimen Handling,
pubmed-meshheading:17549679-Spectrophotometry, Ultraviolet,
pubmed-meshheading:17549679-Tissue Distribution
|
| pubmed:year |
2007
|
| pubmed:articleTitle |
HPLC method for comparative study on tissue distribution in rat after oral administration of salvianolic acid B and phenolic acids from Salvia miltiorrhiza.
|
| pubmed:affiliation |
School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100083, People's Republic of China.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|