Source:http://linkedlifedata.com/resource/pubmed/id/17546037
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
|
| pubmed:dateCreated |
2007-6-29
|
| pubmed:abstractText |
We developed a deep-ultraviolet (UV) microscope capable of imaging cell mitosis and motility at 280 nm for 45 min with minimal UV-induced toxicity, and for 6 h before the onset of visible cell death in cultured human and mouse cells. Combined with computational methods that convert the intensity of each pixel into an estimate of mass, deep-UV microscopy images generate maps of nucleic acid mass, protein mass and fluorescence yield in unlabeled cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
1548-7091
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
4
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
567-9
|
| pubmed:meshHeading |
pubmed-meshheading:17546037-Animals,
pubmed-meshheading:17546037-Cell Movement,
pubmed-meshheading:17546037-Humans,
pubmed-meshheading:17546037-Image Processing, Computer-Assisted,
pubmed-meshheading:17546037-Mice,
pubmed-meshheading:17546037-Microscopy, Ultraviolet,
pubmed-meshheading:17546037-Mitosis,
pubmed-meshheading:17546037-Nucleic Acids,
pubmed-meshheading:17546037-Proteins
|
| pubmed:year |
2007
|
| pubmed:articleTitle |
Nucleic acid and protein mass mapping by live-cell deep-ultraviolet microscopy.
|
| pubmed:affiliation |
WI-MIT BioImaging Center, 500 Technology Square NE47-287, Cambridge, Massachusetts 02142, USA. bzeskind@alum.mit.edu
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|