Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2007-7-3
pubmed:abstractText
Epigenetic and genetic mechanisms can result in large differences in expression levels of the two alleles in a diploid organism. Furthermore, these differences may be critical to phenotypic variations among individuals. In this study, we present a novel procedure and algorithm to precisely and accurately quantitate the relative expression of each allele. This method uses the differential melting properties of DNAs differing at even a single base pair. By referring to the melting characteristics of the two pure alleles, the fractional contribution of the two alleles to any unknown mixture can be mathematically resolved. These methods are highly accurate and precise because each single melting reaction yields multiple data points for analysis. Finally, we discuss how this approach can be used more generally to accurately quantitate gene expression relative to known standards.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17545578-10817754, http://linkedlifedata.com/resource/pubmed/commentcorrection/17545578-10921905, http://linkedlifedata.com/resource/pubmed/commentcorrection/17545578-12627232, http://linkedlifedata.com/resource/pubmed/commentcorrection/17545578-12724780, http://linkedlifedata.com/resource/pubmed/commentcorrection/17545578-12902379, http://linkedlifedata.com/resource/pubmed/commentcorrection/17545578-14962979, http://linkedlifedata.com/resource/pubmed/commentcorrection/17545578-15082756, http://linkedlifedata.com/resource/pubmed/commentcorrection/17545578-15242406, http://linkedlifedata.com/resource/pubmed/commentcorrection/17545578-15499010, http://linkedlifedata.com/resource/pubmed/commentcorrection/17545578-15522875, http://linkedlifedata.com/resource/pubmed/commentcorrection/17545578-15809271, http://linkedlifedata.com/resource/pubmed/commentcorrection/17545578-1671714, http://linkedlifedata.com/resource/pubmed/commentcorrection/17545578-2415964, http://linkedlifedata.com/resource/pubmed/commentcorrection/17545578-7630404, http://linkedlifedata.com/resource/pubmed/commentcorrection/17545578-9467008, http://linkedlifedata.com/resource/pubmed/commentcorrection/17545578-9851976
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1088-9051
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1093-100
pubmed:dateRevised
2011-10-7
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Accurate quantitation of allele-specific expression patterns by analysis of DNA melting.
pubmed:affiliation
Laboratory of Mammalian Genes and Development, National Institute of Child Health and Development, National Institutes of Health, Bethesda, Maryland 20892, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Intramural