Linked Life Data

17545471

Source:http://linkedlifedata.com/resource/pubmed/id/17545471

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2007-9-6
pubmed:abstractText
This study was designed to investigate the developmental expression of endothelial nitric oxide synthase (eNOS) during stem cell differentiation into endothelial cells and to examine the functional status of the newly differentiated endothelial cells. Mouse adult multipotent progenitor cells (MAPCs) were used as the source of stem cells and were induced to differentiate into endothelial cells with vascular endothelial growth factor (VEGF) in serum-free medium. Expression of eNOS in the cells during differentiation was evaluated with real-time PCR, nitric oxide synthase (NOS) activity, and Western blot analysis. It was found that eNOS, but no other NOS, was present in undifferentiated MAPCs. eNOS expression disappeared in the cells immediately after induction of differentiation. However, eNOS expression reoccurred at day 7 during differentiation. Increasing eNOS mRNA, protein content, and activity were observed in the cells at days 14 and 21 during differentiation. The differentiated endothelial cells formed dense capillary networks on growth factor-reduced Matrigel. VEGF-stimulated phosphorylation of extracellular signal-regulated kinase (ERK)-1 and ERK-2 occurred in these cells, which was inhibited by NOS inhibitor N(G)-nitro-L-arginine methyl ester. In conclusion, these data demonstrate that eNOS is present in MAPCs and is dynamically expressed during the differentiation of MAPCs into endothelial cells in vitro.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0363-6135
pubmed:author
pubmed:issnType
Print
pubmed:volume
293
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
H1760-5
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:17545471-Animals, pubmed-meshheading:17545471-Bone Marrow Cells, pubmed-meshheading:17545471-Cell Differentiation, pubmed-meshheading:17545471-Cells, Cultured, pubmed-meshheading:17545471-Down-Regulation, pubmed-meshheading:17545471-Endothelial Cells, pubmed-meshheading:17545471-Endothelium, Vascular, pubmed-meshheading:17545471-Enzyme Inhibitors, pubmed-meshheading:17545471-Extracellular Signal-Regulated MAP Kinases, pubmed-meshheading:17545471-Mice, pubmed-meshheading:17545471-Mitogen-Activated Protein Kinase 1, pubmed-meshheading:17545471-Multipotent Stem Cells, pubmed-meshheading:17545471-NG-Nitroarginine Methyl Ester, pubmed-meshheading:17545471-Nitric Oxide Synthase Type III, pubmed-meshheading:17545471-Phosphorylation, pubmed-meshheading:17545471-Vascular Endothelial Growth Factor A
pubmed:year
2007
pubmed:articleTitle
Endothelial nitric oxide synthase is dynamically expressed during bone marrow stem cell differentiation into endothelial cells.
pubmed:affiliation
Davis Heart & Lung Research Institute, The Ohio State University Medical Center, Columbus, OH 43210, USA. zhenguo.liu@osumc.edu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural