Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
23
pubmed:dateCreated
1992-1-29
pubmed:abstractText
Self-cleavage efficiency of ribozymes derived from hepatitis delta virus (HDV) has been shown to depend on the RNA structure, which in turn may be determined by the length of the considered sequences. Here we describe the construction and functional analysis of a 71 nucleotide-long RNA genomic fragment, Rz71, which carries an 18 nucleotide deletion in a very stable GC-rich stem-loop (stem IV), predicted to be present in several computer-derived secondary structure models. Rz71 is able to undergo self-cleavage under non-denaturing conditions (the t1/2 of the reaction at 37 degrees C is 3 min). The deletion, however, is not neutral, since under the same conditions the non-deleted ribozyme cleaves to 50% in less than 15 sec. Therefore, stem-loop IV seems to play a structural role, not being directly involved in the catalytic reaction, but contributing to the correct positioning of the catalytic core of the HDV ribozyme. Rz71 is the smallest self-cleaving sub-fragment of HDV genomic RNA reported so far.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1754389-1709487, http://linkedlifedata.com/resource/pubmed/commentcorrection/1754389-1893775, http://linkedlifedata.com/resource/pubmed/commentcorrection/1754389-2011192, http://linkedlifedata.com/resource/pubmed/commentcorrection/1754389-2011528, http://linkedlifedata.com/resource/pubmed/commentcorrection/1754389-2183177, http://linkedlifedata.com/resource/pubmed/commentcorrection/1754389-2261458, http://linkedlifedata.com/resource/pubmed/commentcorrection/1754389-2263447, http://linkedlifedata.com/resource/pubmed/commentcorrection/1754389-2398903, http://linkedlifedata.com/resource/pubmed/commentcorrection/1754389-2429192, http://linkedlifedata.com/resource/pubmed/commentcorrection/1754389-2430299, http://linkedlifedata.com/resource/pubmed/commentcorrection/1754389-2436805, http://linkedlifedata.com/resource/pubmed/commentcorrection/1754389-2455816, http://linkedlifedata.com/resource/pubmed/commentcorrection/1754389-2468181, http://linkedlifedata.com/resource/pubmed/commentcorrection/1754389-2482430, http://linkedlifedata.com/resource/pubmed/commentcorrection/1754389-2648383, http://linkedlifedata.com/resource/pubmed/commentcorrection/1754389-2720777, http://linkedlifedata.com/resource/pubmed/commentcorrection/1754389-2915386, http://linkedlifedata.com/resource/pubmed/commentcorrection/1754389-2995921, http://linkedlifedata.com/resource/pubmed/commentcorrection/1754389-3184270, http://linkedlifedata.com/resource/pubmed/commentcorrection/1754389-3684574, http://linkedlifedata.com/resource/pubmed/commentcorrection/1754389-3762705, http://linkedlifedata.com/resource/pubmed/commentcorrection/1754389-6757759, http://linkedlifedata.com/resource/pubmed/commentcorrection/1754389-75123
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6519-25
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Self-cleavage of a 71 nucleotide-long ribozyme derived from hepatitis delta virus genomic RNA.
pubmed:affiliation
GENSET, Paris, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't