Source:http://linkedlifedata.com/resource/pubmed/id/17540853
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
9
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pubmed:dateCreated |
2007-9-7
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pubmed:abstractText |
Human embryonic stem (HES) cells can give rise to cardiomyocytes in vitro. However, whether undifferentiated HES cells also feature a myocardial regenerative capacity after in vivo engraftment has not been established yet. We compared two HES cell lines (HUES-1 and I6) that were specified toward a cardiac lineage by exposure to bone morphogenetic protein-2 (BMP2) and SU5402, a fibroblast growth factor receptor inhibitor. Real-time polymerase chain reaction (PCR) revealed that the cardiogenic inductive factor turned on expression of mesodermal and cardiac genes (Tbx6, Isl1, FoxH1, Nkx2.5, Mef2c, and alpha-actin). Thirty immunosuppressed rats underwent coronary artery ligation and, 2 weeks later, were randomized and received in-scar injections of either culture medium (controls) or BMP2 (+/-SU5402)-treated HES cells. After 2 months, human cells were detected by anti-human lamin immunostaining, and their cardiomyocytic differentiation was evidenced by their expression of cardiac markers by reverse transcription-PCR and immunofluorescence using an anti-beta myosin antibody. No teratoma was observed in hearts or any other organ of the body. The ability of cardiac-specified HES cells to differentiate along the cardiomyogenic pathway following transplantation into infarcted myocardium raises the hope that these cells might become effective candidates for myocardial regeneration.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1549-4918
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pubmed:author |
pubmed-author:AmitMichalM,
pubmed-author:BellamyValérieV,
pubmed-author:BrunevalPatrickP,
pubmed-author:DesnosMichelM,
pubmed-author:DuboisGilbertG,
pubmed-author:HagègeAlbert AAA,
pubmed-author:ItskovitzJosephJ,
pubmed-author:LeschikJuliaJ,
pubmed-author:MenaschéPhilippeP,
pubmed-author:MessasEmmanuelE,
pubmed-author:PucéatMichelM,
pubmed-author:TomescotAndréA
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pubmed:issnType |
Electronic
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2200-5
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pubmed:meshHeading |
pubmed-meshheading:17540853-Animals,
pubmed-meshheading:17540853-Cell Differentiation,
pubmed-meshheading:17540853-Cells, Cultured,
pubmed-meshheading:17540853-Embryonic Stem Cells,
pubmed-meshheading:17540853-Female,
pubmed-meshheading:17540853-Heart,
pubmed-meshheading:17540853-Humans,
pubmed-meshheading:17540853-Myocardial Infarction,
pubmed-meshheading:17540853-Myocardium,
pubmed-meshheading:17540853-Phenotype,
pubmed-meshheading:17540853-Rats,
pubmed-meshheading:17540853-Rats, Wistar,
pubmed-meshheading:17540853-Regeneration,
pubmed-meshheading:17540853-Transplantation, Heterologous
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pubmed:year |
2007
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pubmed:articleTitle |
Differentiation in vivo of cardiac committed human embryonic stem cells in postmyocardial infarcted rats.
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pubmed:affiliation |
Institut National de la Santé et de la Recherche Médicale/Evry University Unité Mixte de Recherche 861, I-Stem, Association Française contre les Myopathies, 5, rue Desbrières, Evry 91030, France.
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pubmed:publicationType |
Journal Article,
Evaluation Studies,
Research Support, N.I.H., Extramural
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