Source:http://linkedlifedata.com/resource/pubmed/id/17540177
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
2007-6-1
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| pubmed:abstractText |
In cells undergoing apoptosis, mitochondrial outer-membrane permeabilization (MOMP) is followed by caspase activation promoted by released cytochrome c. Although caspases mediate the apoptotic phenotype, caspase inhibition is generally not sufficient for survival following MOMP; instead cells undergo a "caspase-independent cell death" (CICD). Thus, MOMP may represent a point of commitment to cell death. Here, we identify glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a critical regulator of CICD. GAPDH-expressing cells preserved their clonogenic potential following MOMP, provided that caspase activation was blocked. GAPDH-mediated protection of cells from CICD involved an elevation in glycolysis and a nuclear function that correlated with and was replaced by an increase in Atg12 expression. Consistent with this, protection from CICD reflected an increase in and a dependence upon autophagy, associated with a transient decrease in mitochondrial mass. Therefore, GAPDH mediates an elevation in glycolysis and enhanced autophagy that cooperate to protect cells from CICD.
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| pubmed:grant | |
| pubmed:commentsCorrections | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0092-8674
|
| pubmed:author |
pubmed-author:BarbryPascalP,
pubmed-author:BeereHelen MHM,
pubmed-author:Bouchier-HayesLisaL,
pubmed-author:ColellAnnaA,
pubmed-author:FitzgeraldPatrickP,
pubmed-author:GreenDouglas RDR,
pubmed-author:Guio-CarrionAnaA,
pubmed-author:LiCindy WeiCW,
pubmed-author:MariBernardB,
pubmed-author:MaurerUlrichU,
pubmed-author:MilastaSandraS,
pubmed-author:NewmeyerDonald DDD,
pubmed-author:RicciJean-EhrlandJE,
pubmed-author:TaitStephenS,
pubmed-author:WaterhouseNigel JNJ
|
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
129
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
983-97
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:17540177-Apoptosis,
pubmed-meshheading:17540177-Autophagy,
pubmed-meshheading:17540177-Caspases,
pubmed-meshheading:17540177-Cell Survival,
pubmed-meshheading:17540177-Cytochromes c,
pubmed-meshheading:17540177-Glyceraldehyde-3-Phosphate Dehydrogenases,
pubmed-meshheading:17540177-HeLa Cells,
pubmed-meshheading:17540177-Humans,
pubmed-meshheading:17540177-Jurkat Cells,
pubmed-meshheading:17540177-Mitochondria,
pubmed-meshheading:17540177-Mitochondrial Membranes,
pubmed-meshheading:17540177-RNA Interference
|
| pubmed:year |
2007
|
| pubmed:articleTitle |
GAPDH and autophagy preserve survival after apoptotic cytochrome c release in the absence of caspase activation.
|
| pubmed:affiliation |
Department of Cell Death and Proliferation, Institut d'Investigacions Biomediques de Barcelona (IIBB-CSIC), IDIBAPS, 08036 Barcelona, Spain.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|