Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
2007-7-26
pubmed:abstractText
Although recombinants based on the attenuated poxvirus vectors MVA and NYVAC are currently in clinical trials, the nature of the genes triggered by these vectors in antigen-presenting cells is poorly characterized. Using microarray technology and various analysis conditions, we compared specific changes in gene expression profiling following MVA and NYVAC infection of immature human monocyte-derived dendritic cells (MDDC). Microarray analysis was performed at 6 h postinfection, since these viruses induced extensive cytopathic effects, rRNA breakdown, and apoptosis at late times postinfection. MVA- and NYVAC-infected MDDC shared upregulation of 195 genes compared to uninfected cells: MVA specifically upregulated 359 genes, and NYVAC upregulated 165 genes. Microarray comparison of NYVAC and MVA infection revealed 544 genes with distinct expression patterns after poxvirus infection and 283 genes specifically upregulated after MVA infection. Both vectors upregulated genes for cytokines, cytokine receptors, chemokines, chemokine receptors, and molecules involved in antigen uptake and processing, including major histocompatibility complex genes. mRNA levels for interleukin 12beta (IL-12beta), beta interferon, and tumor necrosis factor alpha were higher after MVA infection than after NYVAC infection. The expression profiles of transcription factors such as NF-kappaB/Rel and STAT were regulated similarly by both viruses; in contrast, OASL, MDA5, and IRIG-I expression increased only during MVA infection. Type I interferon, IL-6, and Toll-like receptor pathways were specifically induced after MVA infection. Following MVA or NYVAC infection in MDDC, we found similarities as well as differences between these virus strains in the expression of cellular genes with immunological function, which should have an impact when these vectors are used as recombinant vaccines.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-10049946, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-10211965, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-10426996, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-10536144, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-10586075, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-10600608, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-10644837, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-10704355, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-11005852, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-11017102, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-11021596, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-11027812, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-11035028, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-11124948, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-11162614, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-11390620, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-11509172, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-11967338, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-12015460, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-12101391, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-12407409, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-12511874, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-12566418, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-12590567, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-12743306, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-12857909, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-12869693, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-12920112, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-1350676, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-14683453, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-15140980, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-15144573, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-15208624, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-15285707, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-1566575, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-15681458, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-15710891, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-15983379, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-16105838, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-16150517, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-16176847, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-16214130, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-16281988, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-16379000, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-16424203, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-16595141, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-16731942, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-16740766, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-16797201, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-16912297, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-17005676, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-17005686, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-17056088, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-17224219, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-2033387, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-2237434, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-2784004, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-4057358, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-7510087, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-7527085, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-7542414, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-7609028, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-7758109, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-7961637, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-8099586, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-8718576, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-8827214, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-8876137, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-8879125, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-9294135, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-9445029, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-9521319, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-9529248, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-9546783, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-9601507, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-9607847, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-9624003, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-9690454, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-9792841, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-9819417, http://linkedlifedata.com/resource/pubmed/commentcorrection/17537851-9837798
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
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