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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2007-7-3
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pubmed:abstractText |
The cytoskeleton and cytoskeletal motors play a fundamental role in neurotransmitter receptor trafficking, but proteins that link GABA(B) receptors (GABA(B)Rs) to the cytoskeleton have not been described. We recently identified Marlin-1, a protein that interacts with GABA(B)R1. Here, we explore the association of GABA(B)Rs and Marlin-1 to the cytoskeleton using a combination of biochemistry, microscopy and live cell imaging. Our results indicate that Marlin-1 is associated to microtubules and the molecular motor kinesin-I. We demonstrate that a fraction of Marlin-1 is mobile in dendrites of cultured hippocampal neurons and that mobility is microtubule-dependent. We also show that GABA(B)Rs interact robustly with kinesin-I and that intracellular membranes containing GABA(B)Rs are sensitive to treatments that disrupt a protein complex containing Marlin-1, kinesin-I and tubulin. Finally, we report that a kinesin-I mutant severely impairs receptor transport. We conclude that Marlin-1 and kinesin-1 link GABA(B)Rs to the tubulin cytoskeleton in neurons.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1044-7431
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
35
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
501-12
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:17532644-Animals,
pubmed-meshheading:17532644-Antineoplastic Agents,
pubmed-meshheading:17532644-Cells, Cultured,
pubmed-meshheading:17532644-Cytoskeleton,
pubmed-meshheading:17532644-Embryo, Mammalian,
pubmed-meshheading:17532644-Female,
pubmed-meshheading:17532644-Green Fluorescent Proteins,
pubmed-meshheading:17532644-Hippocampus,
pubmed-meshheading:17532644-Kinesin,
pubmed-meshheading:17532644-Mice,
pubmed-meshheading:17532644-Mice, Inbred BALB C,
pubmed-meshheading:17532644-Neurons,
pubmed-meshheading:17532644-Nocodazole,
pubmed-meshheading:17532644-Pregnancy,
pubmed-meshheading:17532644-Protein Transport,
pubmed-meshheading:17532644-RNA-Binding Proteins,
pubmed-meshheading:17532644-Rats,
pubmed-meshheading:17532644-Rats, Sprague-Dawley,
pubmed-meshheading:17532644-Receptors, GABA,
pubmed-meshheading:17532644-Time Factors,
pubmed-meshheading:17532644-Transfection
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pubmed:year |
2007
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pubmed:articleTitle |
Marlin-1 and conventional kinesin link GABAB receptors to the cytoskeleton and regulate receptor transport.
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pubmed:affiliation |
Physiology and Biophysics, ICBM, Faculty of Medicine, Universidad de Chile Independencia 1027, Santiago, Chile.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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